| Autor: |
Zară-Dănceanu CM; Department of Magnetic Materials and Devices, National Institute of Research and Development for Technical Physics, 700050 Iaşi, Romania., Stavilă C; Department of Magnetic Materials and Devices, National Institute of Research and Development for Technical Physics, 700050 Iaşi, Romania.; Faculty of Physics, Alexandru Ioan Cuza University, 700506 Iaşi, Romania., Minuti AE; Department of Magnetic Materials and Devices, National Institute of Research and Development for Technical Physics, 700050 Iaşi, Romania.; Faculty of Physics, Alexandru Ioan Cuza University, 700506 Iaşi, Romania., Lăbușcă L; Department of Magnetic Materials and Devices, National Institute of Research and Development for Technical Physics, 700050 Iaşi, Romania.; County Emergency Hospital Saint Spiridon, Orthopedics and Traumatology Clinic, 700111 Iaşi, Romania., Nastasa V; Faculty of Veterinary Medicine, 'Ion Ionescu de la Brad' University of Life Sciences (IULS), 8 Mihail Sadoveanu Alley, 700489 Iaşi, Romania., Herea DD; Department of Magnetic Materials and Devices, National Institute of Research and Development for Technical Physics, 700050 Iaşi, Romania., Malancus RN; Faculty of Veterinary Medicine, 'Ion Ionescu de la Brad' University of Life Sciences (IULS), 8 Mihail Sadoveanu Alley, 700489 Iaşi, Romania., Ghercă D; Department of Magnetic Materials and Devices, National Institute of Research and Development for Technical Physics, 700050 Iaşi, Romania., Pasca SA; Faculty of Veterinary Medicine, 'Ion Ionescu de la Brad' University of Life Sciences (IULS), 8 Mihail Sadoveanu Alley, 700489 Iaşi, Romania., Chiriac H; Department of Magnetic Materials and Devices, National Institute of Research and Development for Technical Physics, 700050 Iaşi, Romania., Mares M; Faculty of Veterinary Medicine, 'Ion Ionescu de la Brad' University of Life Sciences (IULS), 8 Mihail Sadoveanu Alley, 700489 Iaşi, Romania., Lupu N; Department of Magnetic Materials and Devices, National Institute of Research and Development for Technical Physics, 700050 Iaşi, Romania. |
| Abstrakt: |
(1) Osteoarthritis (OA) is a progressive joint degenerative disease that currently has no cure. Limitations in the development of innovative disease modifying therapies are related to the complexity of the underlying pathogenic mechanisms. In addition, there is the unmet need for efficient drug delivery methods. Magnetic nanoparticles (MNPs) have been proposed as an efficient modality for the delivery of bioactive molecules within OA joints, limiting the side effects associated with systemic delivery. We previously demonstrated MNP's role in increasing cell proliferation and chondrogenesis. In the design of intra-articular therapies for OA, the combined NE-MNP delivery system could provide increased stability and biological effect. (2) Proprietary Fe 3 O 4 MNPs formulated as oil-in-water (O/W) magneto nanoemulsions (MNEs) containing ascorbic acid and dexamethasone were tested for size, stability, magnetic properties, and in vitro biocompatibility with human primary adipose mesenchymal cells (ADSC), cell mobility, and chondrogenesis. In vivo biocompatibility was tested after systemic administration in mice. (3) We report high MNE colloidal stability, magnetic properties, and excellent in vitro and in vivo biocompatibility. By increasing ADSC migration potential and chondrogenesis, MNE carrying dexamethasone and ascorbic acid could reduce OA symptoms while protecting the cartilage layer. |
