Extracellular-Vesicle-Based Cancer Panels Diagnose Glioblastomas with High Sensitivity and Specificity.

Autor: Mut M; Department of Neurosurgery, Faculty of Medicine, Hacettepe University, Ankara 06230, Turkey.; Institute of Neurological Sciences and Psychiatry, Hacettepe University, Ankara 06230, Turkey., Adiguzel Z; TUBİTAK, GEBI, Gebze, Kocaeli 41470, Turkey.; Faculty of Medicine KUTTAM, Koç University, Davutpaşa Street No. 4 Topkapi, Istanbul 34010, Turkey., Cakir-Aktas C; Institute of Neurological Sciences and Psychiatry, Hacettepe University, Ankara 06230, Turkey., Hanalioğlu Ş; Department of Neurosurgery, Faculty of Medicine, Hacettepe University, Ankara 06230, Turkey., Gungor-Topcu G; TUBİTAK, GEBI, Gebze, Kocaeli 41470, Turkey., Kiyga E; TUBİTAK, GEBI, Gebze, Kocaeli 41470, Turkey., Isikay I; Department of Neurosurgery, Faculty of Medicine, Hacettepe University, Ankara 06230, Turkey., Sarac A; TUBİTAK, GEBI, Gebze, Kocaeli 41470, Turkey., Soylemezoglu F; Department of Pathology, Faculty of Medicine, Hacettepe University, Ankara 06230, Turkey., Strobel T; Division of Neuropathology and Neurochemistry, Department of Neurology, Medical University of Vienna, 1090 Vienna, Austria., Ampudia-Mesias E; Division of Hematology and Oncology, Department of Pediatrics, Medical School, University of Minnesota, Minneapolis, MN 55455, USA., Cameron C; Division of Hematology and Oncology, Department of Pediatrics, Medical School, University of Minnesota, Minneapolis, MN 55455, USA., Aslan T; Department of Neurosurgery, Faculty of Medicine, Hacettepe University, Ankara 06230, Turkey., Tekirdas E; Department of Neurosurgery, Faculty of Medicine, Hacettepe University, Ankara 06230, Turkey., Hayran M; Department of Preventive Oncology, Hacettepe University Cancer Institute, Ankara 06230, Turkey., Oguz KK; Department of Radiology, Faculty of Medicine, Hacettepe University, Ankara 06230, Turkey., Henzler C; Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN 55455, USA., Saydam N; ExoMed Diagnostic, Minneapolis, MN 55404, USA., Saydam O; Division of Hematology and Oncology, Department of Pediatrics, Medical School, University of Minnesota, Minneapolis, MN 55455, USA.
Jazyk: angličtina
Zdroj: Cancers [Cancers (Basel)] 2023 Jul 26; Vol. 15 (15). Date of Electronic Publication: 2023 Jul 26.
DOI: 10.3390/cancers15153782
Abstrakt: Glioblastoma is one of the most devastating neoplasms of the central nervous system. This study focused on the development of serum extracellular vesicle (EV)-based glioblastoma tumor marker panels that can be used in a clinic to diagnose glioblastomas and to monitor tumor burden, progression, and regression in response to treatment. RNA sequencing studies were performed using RNA isolated from serum EVs from both patients ( n = 85) and control donors ( n = 31). RNA sequencing results for preoperative glioblastoma EVs compared to control EVs revealed 569 differentially expressed genes (DEGs, 2XFC, FDR < 0.05). By using these DEGs, we developed serum-EV-based biomarker panels for the following glioblastomas: wild-type IDH1 (96% sensitivity/80% specificity), MGMT promoter methylation (91% sensitivity/73% specificity), p53 gene mutation (100% sensitivity/89% specificity), and TERT promoter mutation (89% sensitivity/100% specificity). This is the first study showing that serum-EV-based biomarker panels can be used to diagnose glioblastomas with a high sensitivity and specificity.
Databáze: MEDLINE
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