Storage-related stability and antimicrobial efficacy of bottled, neutral-pH Electrolysed Oxidising Water.

Autor: Krishnan CS; Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand., Lyons KM; Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand., Tompkins GR; Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand., Cannon RD; Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand. Electronic address: richard.cannon@otago.ac.nz.
Jazyk: angličtina
Zdroj: Journal of dentistry [J Dent] 2023 Oct; Vol. 137, pp. 104656. Date of Electronic Publication: 2023 Aug 09.
DOI: 10.1016/j.jdent.2023.104656
Abstrakt: Objectives: Denture stomatitis is prevalent in older people and poses serious health risks. Ready-to-use (RTU) neutral-pH Electrolysed Oxidizing Water (EOW) is an effective environmental disinfectant used in residential care settings and geriatric wards. However, the influence of storage on stability and effectiveness for denture disinfection has not been established. This research investigated the storage-related stability and antimicrobial activity of RTU EOW, and its efficacy against Candida albicans biofilms formed on denture resin.
Methods: The pH, oxidation/reduction potential (mV), available chlorine content (mg/L) and [HOCl] (mM) of RTU EOW (Envirolyte, New Zealand) solutions (n = 22) were measured from bottle opening to 28 days following storage at 4 °C, room temperature (RT) or 37 °C. Staphylococcus aureus and C. albicans cells were incubated in 80% EOW for contact times (CTs) up to 15 min and colony-forming units (cfu) determined. Minimum inhibitory concentrations (MIC 90 EOW-HOCl) after CTs up to five minutes were determined for S. aureus and C. albicans reference strains and clinical isolates. C. albicans-denture resin disc biofilms were assessed after a five-minute CT with undiluted EOW by XTT-metabolic activity assay.
Results: [HOCl] remained stable when RTU EOW was stored at 4 °C or RT for five months after manufacture. One-minute CT resulted in log 10 cfu reductions of >6 for S. aureus and >5 for C. albicans. Mean MIC 90 for five-minute CT was 37 µM (S. aureus) and 54 µM (C. albicans). Undiluted EOW reduced C. albicans biofilm metabolic activity by 86%.
Conclusions: RTU neutral-pH EOW is stable over five-months storage and is an effective denture disinfectant.
Clinical Significance: The efficacy of the RTU neutral EOW against C. albicans isolates and biofilms formed on denture resin surfaces supports its use as a denture disinfectant and can inform future research to assess its potential for preventing denture-related oral Candida infections in the older population, especially in resource-limited communities.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier Ltd.)
Databáze: MEDLINE
Externí odkaz: