Monitoring of monkeypox viral DNA in Prague wastewater.

Autor: Bartáčková J; Department of Water Technology and Environmental Engineering, University of Chemistry and Technology Prague, Czechia., Kouba V; Department of Water Technology and Environmental Engineering, University of Chemistry and Technology Prague, Czechia. Electronic address: vojtech.kouba@vscht.cz., Dostálková A; Department of Biotechnology, University of Chemistry and Technology Prague, Czechia., Čermáková E; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Czechia., Lopez Marin MA; Department of Water Technology and Environmental Engineering, University of Chemistry and Technology Prague, Czechia., Chmel M; Military Health Institute, Military Medical Agency, Czechia; Department of Infectious Diseases, First Faculty of Medicine, Charles University and Military University Hospital Prague, Prague, Czechia., Milanová M; Department of Radiobiology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic., Demnerová K; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Czechia., Rumlová M; Department of Biotechnology, University of Chemistry and Technology Prague, Czechia., Sýkora P; Prazske vodovody a kanalizace, a.s., Czechia., Bartáček J; Department of Water Technology and Environmental Engineering, University of Chemistry and Technology Prague, Czechia., Zdeňková K; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Czechia.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2023 Dec 01; Vol. 902, pp. 166110. Date of Electronic Publication: 2023 Aug 09.
DOI: 10.1016/j.scitotenv.2023.166110
Abstrakt: Monkeypox virus (Mpxv) is a dsDNA virus that has become a global concern for human health in 2022. As both infected people and non-human hosts can shed the virus from their skin, faeces, urine and other body fluids, and the resulting sewage contains viral load representative of the whole population, it is highly promising to detect the spread of monkeypox virus in municipal wastewater. We established a methodology for sewage-based monitoring of Mpxv in Prague and analysed samples (n = 24) already early August-October of 2022 in a municipality with 1.4 million inhabitants that only reported 29 cumulative cases in this period. We isolated Mpxv DNA with the Wizard Enviro Total Nucleic Acid Kit, and thereafter detected Mpxv DNA using the EliGene® Monkeypox RT-PCR Kit. Prague wastewater was positive for Mpxv (in total 9 positive samples in periods with 1-9 new cases per week, coinciding with a weekly incidence of 0.07-0.64 per 100,000 inhabitants. The method for confirmation of wastewater positivity via semi-nested PCR and Sanger sequencing was successfully confirmed on positive controls including Mpxv particles and Mpxv-positive wastewater from the Netherlands. However, for Prague wastewater samples, amplification of Mpxv DNA via semi-semi-nested PCR was unsuccessful. This was probably due to extremely low case count, leading to the amplification of non-target bacterial DNA. Compared to other studies with much higher Mpxv prevalence, we show the outstanding sensitivity of our approach for monitoring the spread of monkeypox using wastewater.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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