A Photopolymerizable Biocompatible Hyaluronic Acid Hydrogel Promotes Early Articular Cartilage Repair in a Minipig Model In Vivo.

Autor: Gao L; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany., Beninatto R; Fidia Farmaceutici S.p.A., Via Ponte della Fabbrica 3/A, Abano Terme (PD), 35031, Italy., Oláh T; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany., Goebel L; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany., Tao K; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany., Roels R; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany., Schrenker S; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany., Glomm J; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany., Venkatesan JK; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany., Schmitt G; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany., Sahin E; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany., Dahhan O; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany., Pavan M; Fidia Farmaceutici S.p.A., Via Ponte della Fabbrica 3/A, Abano Terme (PD), 35031, Italy., Barbera C; Fidia Farmaceutici S.p.A., Via Ponte della Fabbrica 3/A, Abano Terme (PD), 35031, Italy., Lucia AD; Fidia Farmaceutici S.p.A., Via Ponte della Fabbrica 3/A, Abano Terme (PD), 35031, Italy., Menger MD; Institute for Clinical and Experimental Surgery, Saarland University, Kirrberger Straße 100, Building 65 and 66, D-66421, Homburg, Germany., Laschke MW; Institute for Clinical and Experimental Surgery, Saarland University, Kirrberger Straße 100, Building 65 and 66, D-66421, Homburg, Germany., Cucchiarini M; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany., Galesso D; Fidia Farmaceutici S.p.A., Via Ponte della Fabbrica 3/A, Abano Terme (PD), 35031, Italy., Madry H; Center of Experimental Orthopaedics, Saarland University, Kirrberger Straße 100, Building 37, D-66421, Homburg, Germany.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2023 Oct; Vol. 12 (26), pp. e2300931. Date of Electronic Publication: 2023 Aug 18.
DOI: 10.1002/adhm.202300931
Abstrakt: Articular cartilage defects represent an unsolved clinical challenge. Photopolymerizable hydrogels are attractive candidates supporting repair. This study investigates the short-term safety and efficacy of two novel hyaluronic acid (HA)-triethylene glycol (TEG)-coumarin hydrogels photocrosslinked in situ in a clinically relevant large animal model. It is hypothesized that HA-hydrogel-augmented microfracture (MFX) is superior to MFX in enhancing early cartilage repair, and that the molar degree of substitution and concentration of HA affects repair. Chondral full-thickness defects in the knees of adult minipigs are treated with either 1) debridement (No MFX), 2) debridement and MFX, 3) debridement, MFX, and HA hydrogel (30% molar derivatization, 30 mg mL -1 HA; F3) (MFX+F3), and 4) debridement, MFX, and HA hydrogel (40% molar derivatization, 20 mg mL -1 HA; F4) (MFX+F4). After 8 weeks postoperatively, MFX+F3 significantly improves total macroscopic and histological scores compared with all other groups without negative effects, besides significantly enhancing the individual repair parameters "defect architecture," "repair tissue surface" (compared with No MFX, MFX), and "subchondral bone" (compared with MFX). These data indicate that photopolymerizable HA hydrogels enable a favorable metastable microenvironment promoting early chondrogenesis in vivo. This work also uncovers a mechanism for effective HA-augmented cartilage repair by combining lower molar derivatization with higher concentrations.
(© 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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