The effects of human amnion membrane-derived mesenchymal stem cells conditioned medium on ionizing radiation-induced premature ovarian failure and endoplasmic reticulum stress-related apoptosis mechanism.

Autor: Un B; Department of Obstetrics and Gynecology, Adana City Training and Research Hospital, 01370 Adana, Turkey; Department of Histology and Embryology, Medicine Faculty, Zonguldak Bulent Ecevit University, 67600 Zonguldak, Turkey. Electronic address: burakun81@gmail.com., Cetinkaya-Un B; IVF Unit, Adana City Training and Research Hospital, 01370 Adana, Turkey; Department of Histology and Embryology, Medicine Faculty, Zonguldak Bulent Ecevit University, 67600 Zonguldak, Turkey. Electronic address: busracetinkayaun@gmail.com., Akpolat M; Department of Histology and Embryology, Medicine Faculty, Zonguldak Bulent Ecevit University, 67600 Zonguldak, Turkey. Electronic address: meryemakpolat@yahoo.com., Andic F; Department of Radiation Oncology, Medicine Faculty, Cukurova University, 01380 Adana, Turkey. Electronic address: fandic@cu.edu.tr., Yazir Y; Department of Histology and Embryology, Medicine Faculty, Kocaeli University, 41001 Kocaeli, Turkey. Electronic address: yusufhan.yazir@kocaeli.edu.tr.
Jazyk: angličtina
Zdroj: European journal of obstetrics, gynecology, and reproductive biology [Eur J Obstet Gynecol Reprod Biol] 2023 Sep; Vol. 288, pp. 191-197. Date of Electronic Publication: 2023 Aug 06.
DOI: 10.1016/j.ejogrb.2023.08.001
Abstrakt: Objectives: Premature ovarian failure (POF) is defined as the cessation of menstrual periods for at least 4-6 months before the age of 40 years, accompanied by FSH values measuring over 40 IU/L for a month. Radiation therapy, one of the cancer treatment methods, is known to accelerate ovarian aging by reducing and eliminating the number of primordial follicles in the ovarian follicle pool. Ionizing radiation has been reported to cause POF. The objective of this study is to investigate the impact of mesenchymal stem cell conditioned medium (hAMSCs-CM), which is isolated from the amniotic membrane of human placenta, on premature ovarian failure (POF) caused by whole-body irradiation. The study will focus on the ER stress and apoptosis mechanisms in the process.
Study Disayn: A POF model was created by exposing rats to 7 Gy of whole-body irradiation. Serum-free hAMSCs-CM were then administered via the tail vein. Follicle count was performed on the ovaries, and immunohistochemistry was used to determine the expressions of GRP78, CHOP, IRE-1, caspase-12, caspase-9, caspase-3. TUNEL was also carried out, and levels of serum FSH, LH, E2, AMH, and oxidative stress marker 8-OHdG were measured.
Results and Conclusion: The application of hAMSCs-CM has been found to have a positive impact on follicles affected by radiation. After treatment, the number of primordial, primary, secondary, and graafian follicles, which had previously decreased due to radiation, showed an increase. Furthermore, the number of atretic follicles, which had been increasing due to radiation, showed a decrease. ER is one of the targets affected by ionizing radiation. After ionizing radiation, the expressions of ER stress-related markers and apoptosis markers increased in the ovary. After hAMSCs-CM administration, the expressions of these markers and number of TUNEL-positive cells decreased. Following irradiation, anti-mullerian hormone (AMH) and estradiol (E2) levels decreased, while follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels increased. After administration of hAMSCs-CM, AMH and E2 levels increased, while FSH and LH levels decreased. Amnion membrane-derived mesenchymal stem cell conditioned medium can play a therapeutic role in ionizing radiation-induced premature ovarian failure by reducing endoplasmic reticulum stress and apoptosis.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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