The influence of different sugammadex doses on neural tube development in early-stage chick embryos.

Autor: Ayhan A; Department of Anaesthesiology and Reanimation, Baskent University School of Medicine, Ankara, Turkey., Efe E; Department of Histology and Embryology, Baskent University School of Medicine, Ankara, Turkey., Fidan PA; Department of Histology and Embryology, Baskent University School of Medicine, Ankara, Turkey., Efe OE; Department of Pharmacology, Baskent University School of Medicine, Ankara, Turkey., Ates EG; Department of Biostatistics, Baskent University School of Medicine, Ankara, Turkey.; Institutional Big Data Management Unit, Middle East Technical University, Ankara, Turkey., Sahinturk F; Department of Neurological Surgery, Baskent University School of Medicine, Ankara, Turkey., Ayhan S; Department of Neurological Surgery, Baskent University School of Medicine, Ankara, Turkey.
Jazyk: angličtina
Zdroj: Birth defects research [Birth Defects Res] 2023 Oct 15; Vol. 115 (17), pp. 1598-1607. Date of Electronic Publication: 2023 Aug 11.
DOI: 10.1002/bdr2.2237
Abstrakt: Background: Sugammadex is a modified gamma-cyclodextrin that has been developed with the goal of reversing the steroidal neuromuscular blocking agents. The aim of the present study is to investigate the effects of different sugammadex doses on embryologic and neural tube development in an early-stage chick embryo model.
Methods: A total of 100 specific pathogen-free, fertilized domestic chicken eggs were randomly divided into five groups (n = 20, each), and placed in an automatic cycle incubator. The eggs in the "control (C)" group were incubated without administration of any drug till the end of the experiment. Sub-blastodermic administration of 0.9% NaCl as vehicle control (VC) and different doses of sugammadex solutions prepared with the latter [2 mg/mL (LD), 4 mg/mL (MD), 16 mg/mL (HD)] were performed at 30 hr of incubation. All embryos were removed from the eggs at 72 hr when they were expected to reach Hamburger-Hamilton (HH) stages 19-20, then they were fixed, and evaluated histo-morphologically.
Results: Embryonic development was not observed in 11 eggs (1 in C, 1 in VC; 3 in LD, 3 in MD, and 3 in HD). All the developed embryos were compatible with the HH stages 19-20. A neural tube closure defect was detected in one embryo in the HD group. No statistically significant difference was found between the groups in terms of embryonic and neural tube developments.
Conclusions: No significant association was found between the drug and adverse outcomes; however, a trend with dosing was seen. Further studies are required before conclude on safety and extrapolate these results to human beings.
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Databáze: MEDLINE
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