Increased membrane Ca 2+ permeability drives astrocytic Ca 2+ dynamics during neuronal stimulation at excitatory synapses.

Autor:	Hjukse JB; Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway., Puebla MFDL; Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.; Department of Neurology, Neuroclinic, St. Olavs Hospital, Trondheim, Norway., Vindedal GF; Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway., Sprengel R; Department of Neurology, Oslo University Hospital, Oslo, Norway., Jensen V; Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway., Nagelhus EA; Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.; Research Group of Molecular Neurobiology, Max Planck Institute for Medical Research, Heidelberg, Germany., Tang W; Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.; Department of Neurology, Neuroclinic, St. Olavs Hospital, Trondheim, Norway.
Jazyk:	angličtina
Zdroj:	Glia [Glia] 2023 Dec; Vol. 71 (12), pp. 2770-2781. Date of Electronic Publication: 2023 Aug 11.
DOI:	10.1002/glia.24450
Abstrakt:	Astrocytes are intricately involved in the activity of neural circuits; however, their basic physiology of interacting with nearby neurons is not well established. Using two-photon imaging of neurons and astrocytes during higher frequency stimulation of hippocampal CA3-CA1 Schaffer collateral (Scc) excitatory synapses, we could show that increasing levels of released glutamate accelerated local astrocytic Ca 2+ elevation. However, blockage of glutamate transporters did not abolish this astrocytic Ca 2+ response, suggesting that astrocytic Ca 2+ elevation is indirectly associated with an uptake of extracellular glutamate. However, during the astrocytic glutamate uptake, the Na + /Ca 2+ exchanger (NCX) reverse mode was activated, and mediated extracellular Ca 2+ entry, thereby triggering the internal release of Ca 2+ . In addition, extracellular Ca 2+ entry via membrane P2X receptors further facilitated astrocytic Ca 2+ elevation via ATP binding. These findings suggest a novel mechanism of activity induced Ca 2+ permeability increases of astrocytic membranes, which drives astrocytic responses during neuronal stimulation of CA3-CA1 Scc excitatory synapses. (© 2023 The Authors. GLIA published by Wiley Periodicals LLC.)
Databáze:	MEDLINE
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