A multi-omic Nicotiana benthamiana resource for fundamental research and biotechnology.

Autor: Ranawaka B; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia.; ARC Centre of Excellence for Plant Success in Nature & Agriculture, Brisbane, Queensland, Australia., An J; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia. j.an@qut.edu.au.; ARC Centre of Excellence for Plant Success in Nature & Agriculture, Brisbane, Queensland, Australia. j.an@qut.edu.au., Lorenc MT; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia., Jung H; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia.; Centre for Animal Science, Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, Brisbane, Queensland, Australia., Sulli M; Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Casaccia Research Centre, Rome, Italy., Aprea G; Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Casaccia Research Centre, Rome, Italy., Roden S; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia.; ARC Centre of Excellence for Plant Success in Nature & Agriculture, Brisbane, Queensland, Australia., Llaca V; Genomics Technologies, Corteva Agriscience, Johnston, IA, USA., Hayashi S; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia.; ARC Centre of Excellence for Plant Success in Nature & Agriculture, Brisbane, Queensland, Australia., Asadyar L; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia.; ARC Centre of Excellence for Plant Success in Nature & Agriculture, Brisbane, Queensland, Australia., LeBlanc Z; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia., Ahmed Z; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia.; ARC Centre of Excellence for Plant Success in Nature & Agriculture, Brisbane, Queensland, Australia., Naim F; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia.; Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, Bentley, Western Australia, Australia., de Campos SB; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia., Cooper T; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia., de Felippes FF; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia., Dong P; State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China., Zhong S; State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China., Garcia-Carpintero V; Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas (CSIC), Universidad Politècnica de Valencia, Valencia, Spain., Orzaez D; Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas (CSIC), Universidad Politècnica de Valencia, Valencia, Spain., Dudley KJ; School of Biology and Environmental Science, Queensland University of Technology (QUT), Brisbane, Queensland, Australia.; QUT Central Analytical Research Facility, Queensland University of Technology (QUT), Brisbane, Queensland, Australia., Bombarely A; Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas (CSIC), Universidad Politècnica de Valencia, Valencia, Spain.; Università degli Studi di Milano, Milan, Italy., Bally J; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia.; ARC Centre of Excellence for Plant Success in Nature & Agriculture, Brisbane, Queensland, Australia., Winefield C; ARC Centre of Excellence for Plant Success in Nature & Agriculture, Brisbane, Queensland, Australia. chris.winefield@lincoln.ac.nz.; Department of Wine Food and Molecular Biosciences, Lincoln University, Lincoln, New Zealand. chris.winefield@lincoln.ac.nz., Giuliano G; Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), Casaccia Research Centre, Rome, Italy., Waterhouse PM; Centre for Agriculture and the Bioeconomy, Queensland University of Technology (QUT), Brisbane, Queensland, Australia. peter.waterhouse@qut.edu.au.; ARC Centre of Excellence for Plant Success in Nature & Agriculture, Brisbane, Queensland, Australia. peter.waterhouse@qut.edu.au.
Jazyk: angličtina
Zdroj: Nature plants [Nat Plants] 2023 Sep; Vol. 9 (9), pp. 1558-1571. Date of Electronic Publication: 2023 Aug 10.
DOI: 10.1038/s41477-023-01489-8
Abstrakt: Nicotiana benthamiana is an invaluable model plant and biotechnology platform with a ~3 Gb allotetraploid genome. To further improve its usefulness and versatility, we have produced high-quality chromosome-level genome assemblies, coupled with transcriptome, epigenome, microRNA and transposable element datasets, for the ubiquitously used LAB strain and a related wild accession, QLD. In addition, single nucleotide polymorphism maps have been produced for a further two laboratory strains and four wild accessions. Despite the loss of five chromosomes from the ancestral tetraploid, expansion of intergenic regions, widespread segmental allopolyploidy, advanced diploidization and evidence of recent bursts of Copia pseudovirus (Copia) mobility not seen in other Nicotiana genomes, the two subgenomes of N. benthamiana show large regions of synteny across the Solanaceae. LAB and QLD have many genetic, metabolic and phenotypic differences, including disparate RNA interference responses, but are highly interfertile and amenable to genome editing and both transient and stable transformation. The LAB/QLD combination has the potential to be as useful as the Columbia-0/Landsberg errecta partnership, utilized from the early pioneering days of Arabidopsis genomics to today.
(© 2023. The Author(s).)
Databáze: MEDLINE
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