NvPrdm14d-expressing neural progenitor cells contribute to non-ectodermal neurogenesis in Nematostella vectensis.

Autor: Lemaître QIB; Michael Sars Centre, University of Bergen, Thormøhlensgate 55, 5006, Bergen, Norway., Bartsch N; Michael Sars Centre, University of Bergen, Thormøhlensgate 55, 5006, Bergen, Norway.; Department for Biological Sciences, University of Bergen, Thormøhlensgate 55, 5006, Bergen, Norway., Kouzel IU; Michael Sars Centre, University of Bergen, Thormøhlensgate 55, 5006, Bergen, Norway., Busengdal H; Michael Sars Centre, University of Bergen, Thormøhlensgate 55, 5006, Bergen, Norway., Richards GS; Michael Sars Centre, University of Bergen, Thormøhlensgate 55, 5006, Bergen, Norway., Steinmetz PRH; Michael Sars Centre, University of Bergen, Thormøhlensgate 55, 5006, Bergen, Norway., Rentzsch F; Michael Sars Centre, University of Bergen, Thormøhlensgate 55, 5006, Bergen, Norway. fabian.rentzsch@uib.no.; Department for Biological Sciences, University of Bergen, Thormøhlensgate 55, 5006, Bergen, Norway. fabian.rentzsch@uib.no.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Aug 10; Vol. 14 (1), pp. 4854. Date of Electronic Publication: 2023 Aug 10.
DOI: 10.1038/s41467-023-39789-4
Abstrakt: Neurogenesis has been studied extensively in the ectoderm, from which most animals generate the majority of their neurons. Neurogenesis from non-ectodermal tissue is, in contrast, poorly understood. Here we use the cnidarian Nematostella vectensis as a model to provide new insights into the molecular regulation of non-ectodermal neurogenesis. We show that the transcription factor NvPrdm14d is expressed in a subpopulation of NvSoxB(2)-expressing endodermal progenitor cells and their NvPOU4-expressing progeny. Using a new transgenic reporter line, we show that NvPrdm14d-expressing cells give rise to neurons in the body wall and in close vicinity of the longitudinal retractor muscles. RNA-sequencing of NvPrdm14d::GFP-expressing cells and gene knockdown experiments provide candidate genes for the development and function of these neurons. Together, the identification of a population of endoderm-specific neural progenitor cells and of previously undescribed putative motoneurons in Nematostella provide new insights into the regulation of non-ectodermal neurogenesis.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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