Ascending renal infection following experimental candiduria by Candida tropicalis in immunocompromised mice.

Autor: Capote-Bonato FG; Postgraduate Program in Animal Science with Emphasis on Bioactive Products, Paranaense University (UNIPAR), Praça Mascarenhas de Moraes, 4282-Centro, 87502-210, Umuarama, Paraná, Brazil. Electronic address: francieli.bonato@edu.unipar.br., Bonato DV; Postgraduate Program in Animal Science with Emphasis on Bioactive Products, Paranaense University (UNIPAR), Praça Mascarenhas de Moraes, 4282-Centro, 87502-210, Umuarama, Paraná, Brazil., Ayer IM; Department of Veterinary Medicine, Franca University, São Paulo, Brazil., Silva de Lima C; Department of Veterinary Medicine, Franca University, São Paulo, Brazil., Magalhães LF; Department of Veterinary Medicine, Franca University, São Paulo, Brazil., Spada CA; Postgraduate Program in Animal Science with Emphasis on Bioactive Products, Paranaense University (UNIPAR), Praça Mascarenhas de Moraes, 4282-Centro, 87502-210, Umuarama, Paraná, Brazil., Magalhães GM; Federal Institute of the South of Minas, Brazil., de Mattos Junior E; Department of Veterinary Medicine, Franca University, São Paulo, Brazil., Maia Teixeira PP; Department of Veterinary Medicine (DVM), Federal University of Pará, Pará, Brazil., Negri M; Department of Clinical Analysis, Medical Mycology Division, State University of Maringá, Paraná, Brazil., Crivellenti LZ; Department of Veterinary Medicine (DVM), Federal University of Uberlândia, Minas Gerais, Brazil., Estivalet Svidzinski TI; Department of Clinical Analysis, Medical Mycology Division, State University of Maringá, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2023 Oct; Vol. 183, pp. 106295. Date of Electronic Publication: 2023 Aug 08.
DOI: 10.1016/j.micpath.2023.106295
Abstrakt: The present study evaluated renal infection resulting from the implantation of C. tropicalis in the bladder of immunosuppressed mice. Yeasts were implanted in two manners: planktonic and via preformed biofilm on a small catheter fragment (SCF). Renal histopathology and cultures was performed 72 and 144 h after cystotomy was carried out in mice from three groups: group I contained non-contaminated mice implanted with a sterile SCF; group II mice received a sterile SCF plus a yeast suspension containing 1 × 10 7 yeasts/mL in a planktonic form; group III mice were implanted with a SCF containing preformed C. tropicalis biofilm. Viable yeasts were found in the kidneys of mice from both groups II and III. However, after 72 h the planktonic cells (group II) invaded more quickly than the sessile cells (group III). Over a longer period (144 h), group III exhibited a more invasive infection (50% of the animals presented renal infection and the renal fungal load was 3.2 log10 CFU/g tissue) than in group II, where yeasts were not found. C. tropicalis introduced into the bladder in two ways (in planktonic or biofilm form) were able to reach the kidney and establish a renal fungal infection, causing interstitial disorders. The data of the present study therefore support the hypothesis of an ascending pathway for renal infections by C. tropicalis. Furthermore, the biofilm resulted in a greater and progressive risk of renal infection, attributed to the slow detachment of the yeasts.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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