Cladosporium sphaerospermum extract inhibits quorum sensing associated virulence factors of Serratia marcescens .

Autor:	Liu DR; Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Pharmaceutical Sciences, Hainan University, Haikou, 570228, China., Yan QX; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Xiamen, 361005, China., Zou ZB; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Xiamen, 361005, China., Xie CL; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Xiamen, 361005, China., Yang XW; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Xiamen, 361005, China., Jia AQ; The Central Lab, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, 570311, China.
Jazyk:	angličtina
Zdroj:	Biofilm [Biofilm] 2023 Aug 01; Vol. 6, pp. 100146. Date of Electronic Publication: 2023 Aug 01 (Print Publication: 2023).
DOI:	10.1016/j.bioflm.2023.100146
Abstrakt:	Serratia marcescens is now becoming a propensity for its highly antimicrobial-resistant clinical infections. Currently, it provides a novel strategy to prevent and control microbial infection by regulating S. marcescens quorum sensing (QS). Deep-sea-derived fungi are rich in QS bioactive constituents. In this work, the extracts from Cladosporium sphaerospermum SCSGAF0054 showed potent QS-related virulence factors and biofilm-inhibiting activities against S. marcescens NJ01 . The swimming motility and multiple virulence factors such as prodigiosin, exopolysaccharide (EPS), lipase, protease and hemolysin were moderately inhibited by the extracts at varied concentrations. The confocal laser scanning microscope (CLSM) and scanning electron microscope (SEM) images revealed that C. sphaerospermum extracts moderately arrested biofilm formation and cell viability. Further, real-time quantitative PCR (RT-qPCR) analysis revealed that expressions of genes associated with virulence factors, flhD , fimA , fimC , bsmA , bsmB , pigA , pigC , and shlA , were significantly down-regulated compared with control. In addition, the extracts combined with imipenem inhibited the QS system of S. marcescens NJ01, disrupted its preformed biofilm, released the intra-biofilm bacteria and killed the bacteria gradually. Therefore, the extracts combined with imipenem can partially restore bacterial drug sensitivity. These results suggest that the extracts from SCSGAF0054 effectively interfere with the QS system to treat S. marcescens infection alone or combining with classical antimicrobial drugs. Competing Interests: The authors declare have no competing financial interests. (© 2023 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu