Purification of Retinal Ganglion Cells from Differentiation Through Adult via Immunopanning and Low-Pressure Flow Cytometry.

Autor:	Riordan SM; Department of Ophthalmology and Department of Biomedical Sciences, University of Missouri-Kansas City, Kansas City, MO, USA., Aladdad AM; Department of Ophthalmology and Department of Biomedical Sciences, University of Missouri-Kansas City, Kansas City, MO, USA., McLoughlin KJ; Department of Ophthalmology and Department of Biomedical Sciences, University of Missouri-Kansas City, Kansas City, MO, USA.; Wellcome Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, UK., Kador KE; Department of Ophthalmology and Department of Biomedical Sciences, University of Missouri-Kansas City, Kansas City, MO, USA. kadork@umkc.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2708, pp. 11-24.
DOI:	10.1007/978-1-0716-3409-7_2
Abstrakt:	The isolation and culturing of rodent retinal ganglion cells (RGC) is a key step in studying the function and cellular response of this crucial cell type. Typical methods used for isolation of RGCs include immunopanning or magnetic bead separation with antibodies targeting RGC specific protein markers. However, in developmental research, many of the most common markers, such as Thy-1, are not expressed in early stages of development. To help study these crucial early stage RGCs, we have developed a novel method that utilizes a transgenic mouse with a GFP tag on the protein BRN3 and a low-pressure fluorescence-activated cell sorter (FACS) system. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu