Isolation and Culture of Primary Retinal Ganglion Cells from Rodent Retina.

Autor:	Durmaz E; School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK., Kutnyanszky M; School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK., Mead B; School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK. MeadB@cardiff.ac.uk.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2708, pp. 1-10.
DOI:	10.1007/978-1-0716-3409-7_1
Abstrakt:	Primary retinal ganglion cell (RGC) cultures are widely used for evaluating the neuroprotective and neurogenic effects of candidate compounds. The axons of RGCs make up the optic nerve and are responsible for transmitting electrochemical signals to the brain. As the retina is an outgrowth of the brain, both it and the optic nerve are part of the central nervous system (CNS). In the process of culturing RGC, the eye and retina are dissected, meaning the RGC, disconnected from the brain, degenerate without intervention due to the traumatic damage they have endured. Therefore, this in vitro model is invaluable for investigating the CNS therapeutics. Here, we present a protocol for the isolation and culture of primary RGCs from rodent retina. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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