Treatment strategies with vitamin E and C in autologous intraperitoneal ovarian transplantation and its impact on ovarian surface epithelium and follicle reserve.

Autor: Sapmaz T; University of Health Sciences, Hamidiye Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey. Electronic address: tansel.sapmaz@sbu.edu.tr., Kale E; University of Health Sciences, Hamidiye Faculty of Medicine, Department of Medical Biochemistry, Istanbul, Turkey., Pence ME; University of Health Sciences, Istanbul Umraniye Training and Research Hospital, Department of Emergency Medicine, Istanbul, Turkey., Sevgin K; University of Health Sciences, Hamidiye Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey., Tekayev M; University of Health Sciences, Hamidiye Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey., Topkaraoglu S; University of Health Sciences, Hamidiye Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey., Basol G; University of Health Sciences, Lutfi Kirdar City Hospital, Department of Obstetrics and Gynecology, Istanbul, Turkey., Yilmaz MB; University of Health Sciences, Zeynep Kamil Woman's and Children Research and Training Hospital, Istanbul, Turkey., Sapmaz E; University of Health Sciences, Adana City Hospital, Department of Obstetrics and Gynecology, Adana, Turkey., Irkorucu O; University of Sharjah, College of Medicine, Department of Clinical Sciences, Sharjah, United Arab Emirates.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2023 Oct 15; Vol. 677, pp. 81-87. Date of Electronic Publication: 2023 Aug 05.
DOI: 10.1016/j.bbrc.2023.08.002
Abstrakt: The objective of this study was to assess the impact of Vitamin E (Vit E) and Vitamin C (Vit C) on markers of the oxidant-antioxidant system, ovarian follicle reserves, and the surface epithelium in autologous intraperitoneal ovarian transplantation conducted in rats. The study aimed to investigate how these antioxidants influence various aspects related to transplantation outcomes, including oxidative stress markers, the preservation of follicle reserves, and the condition of the surface epithelium. A total of 20 adult female Wistar Albino rats were included in the study and randomly assigned to four different groups. Group 1, consisting of 5 rats, served as the control group and underwent a surgical procedure where their abdomens were opened and closed without any further intervention. Group 2, also consisting of 5 rats, underwent ovarian transplantation. In Group 3, comprising 5 rats, an intraperitoneal (IP) administration of 20 mg/kg body weight (b.w.) of Vitamin E (Vit E) was given 15 min prior to ovarian transplantation. Lastly, in Group 4, which included 5 rats, an IP administration of 50 mg/kg body weight (b.w.) of Vitamin C (Vit C) was given 15 min before ovarian transplantation. Vaginal cytology was performed in order to monitor the estrus phase in the rats. Biochemically, tissue and serum malondialdehyde (MDA) levels and erythrocyte superoxide dismutase (SOD) levels were measured. Histopathologically, the number of dysplastic changes in the ovarian surface epithelium and primordial, primary, secondary, Graaffian, and atretic follicles were examined. Dysplastic changes in the surface epithelium of Group 2 were found to be significantly higher than in Group 1 and 4 (p < 0.02). In Group 2, the ovarian follicle reserves (primordial, primary, secondary, and Graaffian follicles) were significantly lower than in other groups (p < 0.02). In addition, a significant decrease in SOD levels was found in Group 2 compared to other groups (p < 0.02). The study showed that Vit E and Vit C in autologous intraperitoneal ovarian transplantation preserved the ovarian follicle reserve. Vit C was found to be more effective than Vit E.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: