Propofol Suppresses LPS-induced BBB Damage by Regulating miR-130a-5p/ZO-1 Axis.

Autor: Gan N; Department of Anesthesiology, The Sixth People's Hospital Affiliated to the School of Medicine of Shanghai Jiaotong University, 600 Yishan Road, Shanghai, 200233, China., Zhou Y; Department of Anesthesiology, The Sixth People's Hospital Affiliated to the School of Medicine of Shanghai Jiaotong University, 600 Yishan Road, Shanghai, 200233, China., Li J; Department of Anesthesiology, The Sixth People's Hospital Affiliated to the School of Medicine of Shanghai Jiaotong University, 600 Yishan Road, Shanghai, 200233, China., Wang A; Department of Anesthesiology, The Sixth People's Hospital Affiliated to the School of Medicine of Shanghai Jiaotong University, 600 Yishan Road, Shanghai, 200233, China., Cao Y; Department of Anesthesiology, The Sixth People's Hospital Affiliated to the School of Medicine of Shanghai Jiaotong University, 600 Yishan Road, Shanghai, 200233, China. sublimate999@sina.com.
Jazyk: angličtina
Zdroj: Molecular biotechnology [Mol Biotechnol] 2024 Aug; Vol. 66 (8), pp. 2007-2015. Date of Electronic Publication: 2023 Aug 09.
DOI: 10.1007/s12033-023-00835-7
Abstrakt: The blood-brain barrier (BBB) is a highly selective semi-permeable barrier that separates circulating blood from the extracellular fluid of the brain and central nervous system, which is crucial for maintaining brain homeostasis. This study aimed to explore the role of propofol in BBB damage and further evaluate the underlying molecular mechanism. Lipopolysaccharide (LPS) was administered to mice to create an in vivo BBB damage mice model. Additionally, hCMEC/D3 cells as brain microvascular endothelial cells (BMECs) were treated with LPS to establish the in vitro BBB damage cell model. Subsequently, propofol was used for the BBB damage model. Evans blue staining and fluorescein sodium were utilized in the in vivo experiments to demonstrate BBB leakage and BBB permeability. Cell counting kit-8 (CCK-8) assay was used to assess cell viability and the trans-endothelial electrical resistance (TEER) value was measured using an epithelial voltmeter. Furthermore, enzyme-linked immunosorbent assay was performed to measure the levels of the inflammatory cytokines such as interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α). The levels of miR-130a-5p and zonula occludens-1 (ZO-1) in brain tissues and cells were detected using reverse transcription-quantitative polymerase chain reaction, western blot, or immunofluorescence staining. Furthermore, a dual-luciferase reporter assay was used to demonstrate the association between miR-130a-5p and ZO-1. Propofol treatment suppressed BBB leakage, the amount of fluorescein sodium, and the levels of IL-1β and TNF-α in the LPS-induced BBB damage mice model. Meanwhile, propofol treatment increased the TEER value in the LPS-induced hCMEC/D3 cells. Additionally, propofol treatment significantly down-regulated miR-130a-5p and up-regulated ZO-1. More importantly, miR-130a-5p directly targeted ZO-1 and negatively regulated ZO-1 expression in hCMEC/D3 cells. Furthermore, miR-130a-5p mimic partially reversed the effect of propofol on the TEER value and the levels of inflammatory cytokines such as IL-1β and TNF-α in the LPS-induced hCMEC/D3 cells. Propofol suppressed LPS-induced BBB damage by regulating miR-130a-5p/ZO-1 axis. These findings suggested a potentially effective treatment approach for BBB damage.
(© 2023. The Author(s).)
Databáze: MEDLINE
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