A modified BG-Sentinel trap equipped with FTA card as a novel tool for mosquito-borne disease surveillance: a field test for flavivirus detection.

Autor: Manzi S; Dipartimento di Sanità Pubblica e Malattie Infettive, Sapienza Università di Roma, Rome, Italy., Nelli L; School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Glasgow, UK., Fortuna C; Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, Italy., Severini F; Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, Italy., Toma L; Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, Italy., Di Luca M; Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, Italy., Michelutti A; Istituto Zooprofilattico Sperimentale Delle Venezie, Legnaro, Italy., Bertola M; Istituto Zooprofilattico Sperimentale Delle Venezie, Legnaro, Italy., Gradoni F; Istituto Zooprofilattico Sperimentale Delle Venezie, Legnaro, Italy., Toniolo F; Istituto Zooprofilattico Sperimentale Delle Venezie, Legnaro, Italy., Sgubin S; Istituto Zooprofilattico Sperimentale Delle Venezie, Legnaro, Italy., Lista F; Istituto di Scienze Biomediche Della Difesa, Rome, Italy., Pazienza M; Stato Maggiore Della Difesa, Rome, Italy., Montarsi F; Istituto Zooprofilattico Sperimentale Delle Venezie, Legnaro, Italy., Pombi M; Dipartimento di Sanità Pubblica e Malattie Infettive, Sapienza Università di Roma, Rome, Italy. marco.pombi@uniroma1.it.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Aug 08; Vol. 13 (1), pp. 12840. Date of Electronic Publication: 2023 Aug 08.
DOI: 10.1038/s41598-023-39857-1
Abstrakt: Early detection of pathogens in vectors is important in preventing the spread of arboviral diseases, providing a timely indicator of pathogen circulation before outbreaks occur. However, entomological surveillance may face logistical constraints, such as maintaining the cold chain, and resource limitations, such as the field and laboratory workload of mosquito processing. We propose an FTA card-based trapping system that aims to simplify both field and laboratory phases of arbovirus surveillance. We modified a BG-Sentinel trap to include a mosquito collection chamber and a sugar feeding source through an FTA card soaked in a long-lasting viscous solution of honey and hydroxy-cellulose hydrogel. The FTA card ensures environmental preservation of nucleic acids, allowing continuous collection and feeding activity of specimens for several days and reducing the effort required for viral detection. We tested the trap prototype during two field seasons (2019 and 2021) in North-eastern Italy and compared it to CDC-CO 2 trapping applied in West Nile and Usutu virus regional surveillance. Collections by the BG-FTA approach detected high species diversity, including Culex pipiens, Aedes albopictus, Culex modestus, Anopheles maculipennis sensu lato and Ochlerotatus caspius. When used for two-days sampling, the BG-FTA trap performed equally to CDC also for the WNV-major vector Cx. pipiens. The FTA cards detected both WNV and USUV, confirming the reliability of this novel approach to detect viral circulation in infectious mosquitoes. We recommend this surveillance approach as a particularly useful alternative in multi-target surveillance, for sampling in remote areas and in contexts characterized by high mosquito densities and diversity.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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