RNA in Municipal Wastewater Reveals Magnitudes of COVID-19 Outbreaks across Four Waves Driven by SARS-CoV-2 Variants of Concern.

Autor:	Xie Y; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada., Challis JK; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada., Oloye FF; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada., Asadi M; Department of Civil, Geological and Environmental Engineering, College of Engineering, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5A9, Canada., Cantin J; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada., Brinkmann M; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada.; School of Environment and Sustainability, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada.; Global Institute for Water Security, University of Saskatchewan, Saskatoon, Saskatchewan S7N 3H5, Canada., McPhedran KN; Department of Civil, Geological and Environmental Engineering, College of Engineering, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5A9, Canada.; Global Institute for Water Security, University of Saskatchewan, Saskatoon, Saskatchewan S7N 3H5, Canada., Hogan N; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada.; College of Agriculture and Bioresources, Department of Animal and Poultry Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5A8, Canada., Sadowski M; Wastewater Treatment Plant, Saskatoon Water Department, City of Saskatoon, Saskatoon, Saskatchewan S7M 1X5, Canada., Jones PD; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada.; School of Environment and Sustainability, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada., Landgraff C; Division of Enteric Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2, Canada.; Food Science Department, University of Guelph, Guelph, Ontario N1G 2W1, Canada., Mangat C; Antimicrobial Resistance and Nosocomial Infections, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba R3E 3R2, Canada., Servos MR; Department of Biology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada., Giesy JP; Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B3, Canada.; Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4, Canada.; Department of Environmental Sciences, Baylor University, Waco, Texas 76706, United States.; Department of Zoology and Center for Integrative Toxicology, Michigan State University, East Lansing, Michigan 48824, United States.
Jazyk:	angličtina
Zdroj:	ACS ES&T water [ACS ES T Water] 2022 Feb 23; Vol. 2 (11), pp. 1852-1862. Date of Electronic Publication: 2022 Feb 23 (Print Publication: 2022).
DOI:	10.1021/acsestwater.1c00349
Abstrakt:	There are no standardized protocols for quantifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in wastewater to date, especially for population normalization. Here, a pipeline was developed, applied, and assessed to quantify SARS-CoV-2 and key variants of concern (VOCs) RNA in wastewater at Saskatoon, Canada. Normalization approaches using recovery ratio and extraction efficiency, wastewater parameters, or population indicators were assessed by comparing to daily numbers of new cases. Viral load was positively correlated with daily new cases reported in the sewershed. Wastewater surveillance (WS) had a lead time of approximately 7 days, which indicated surges in the number of new cases. WS revealed the variant α and δ driving the third and fourth wave, respectively. The adjustment with the recovery ratio and extraction efficiency improved the correlation between viral load and daily new cases. Normalization of viral concentration to concentrations of the artificial sweetener acesulfame K improved the trend of viral load during the Christmas and New Year holidays when populations were dynamic and variable. Acesulfame K performed better than pepper mild mottle virus, creatinine, and ammonia for population normalization. Hence, quality controls to characterize recovery ratios and extraction efficiencies and population normalization with acesulfame are promising for precise WS programs supporting decision-making in public health. Competing Interests: The authors declare no competing financial interest. (© 2022 American Chemical Society.)
Databáze:	MEDLINE
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