Quantitative Reverse Transcription PCR Surveillance of SARS-CoV-2 Variants of Concern in Wastewater of Two Counties in Texas, United States.

Autor:	Langan LM; Department of Environmental Science, Baylor University, One Bear Place #97266, Waco, Texas 76798, United States.; Center for Reservoir and Aquatic Systems Research, Baylor University, One Bear Place #97178, Waco, Texas 76798, United States., O'Brien M; Department of Environmental Science, Baylor University, One Bear Place #97266, Waco, Texas 76798, United States.; Center for Reservoir and Aquatic Systems Research, Baylor University, One Bear Place #97178, Waco, Texas 76798, United States.; Department of Public Health, Baylor University, One Bear Place #97343, Waco, Texas 76798, United States., Lovin LM; Department of Environmental Science, Baylor University, One Bear Place #97266, Waco, Texas 76798, United States.; Center for Reservoir and Aquatic Systems Research, Baylor University, One Bear Place #97178, Waco, Texas 76798, United States., Scarlett KR; Department of Environmental Science, Baylor University, One Bear Place #97266, Waco, Texas 76798, United States.; Center for Reservoir and Aquatic Systems Research, Baylor University, One Bear Place #97178, Waco, Texas 76798, United States., Davis H; Department of Environmental Science, Baylor University, One Bear Place #97266, Waco, Texas 76798, United States.; Center for Reservoir and Aquatic Systems Research, Baylor University, One Bear Place #97178, Waco, Texas 76798, United States., Henke AN; Department of Environmental Science, Baylor University, One Bear Place #97266, Waco, Texas 76798, United States.; Center for Reservoir and Aquatic Systems Research, Baylor University, One Bear Place #97178, Waco, Texas 76798, United States.; Department of Biology, Baylor University, One Bear Place #97388, Waco, Texas 76798, United States., Seidel SE; Center for Health Statistics, Texas Department of State Health Services, Austin, Texas 78756, United States., Archer N; Environmental Epidemiology and Disease Registries Section, Texas Department of State Health Services, Austin, Texas 78756, United States., Lawrence E; Environmental Epidemiology and Disease Registries Section, Texas Department of State Health Services, Austin, Texas 78756, United States., Norman RS; Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, 921 Assembly Street Columbia, South Carolina 29208, United States., Bojes HK; Environmental Epidemiology and Disease Registries Section, Texas Department of State Health Services, Austin, Texas 78756, United States., Brooks BW; Department of Environmental Science, Baylor University, One Bear Place #97266, Waco, Texas 76798, United States.; Center for Reservoir and Aquatic Systems Research, Baylor University, One Bear Place #97178, Waco, Texas 76798, United States.; Department of Public Health, Baylor University, One Bear Place #97343, Waco, Texas 76798, United States.
Jazyk:	angličtina
Zdroj:	ACS ES&T water [ACS ES T Water] 2022 Jul 06; Vol. 2 (11), pp. 2211-2224. Date of Electronic Publication: 2022 Jul 06 (Print Publication: 2022).
DOI:	10.1021/acsestwater.2c00103
Abstrakt:	After its emergence in late November/December 2019, the severe acute respiratory syndrome coronavirus 2 virus (SARS-CoV-2) rapidly spread globally. Recognizing that this virus is shed in feces of individuals and that viral RNA is detectable in wastewater, testing for SARS-CoV-2 in sewage collections systems has allowed for the monitoring of a community's viral burden. Over a 9 month period, the influents of two regional wastewater treatment facilities were concurrently examined for wild-type SARS-CoV-2 along with variants B.1.1.7 and B.1.617.2 incorporated as they emerged. Epidemiological data including new confirmed COVID-19 cases and associated hospitalizations and fatalities were tabulated within each location. RNA from SARS-CoV-2 was detectable in 100% of the wastewater samples, while variant detection was more variable. Quantitative reverse transcription PCR (RT-qPCR) results align with clinical trends for COVID-19 cases, and increases in COVID-19 cases were positively related with increases in SARS-CoV-2 RNA load in wastewater, although the strength of this relationship was location specific. Our observations demonstrate that clinical and wastewater surveillance of SARS-CoV-2 wild type and constantly emerging variants of concern can be combined using RT-qPCR to characterize population infection dynamics. This may provide an early warning for at-risk communities and increases in COVID-19 related hospitalizations. Competing Interests: The authors declare no competing financial interest. (© 2022 American Chemical Society.)
Databáze:	MEDLINE
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