| Autor: |
Shrestha P; Enrich Biosystems Inc., 21 Business Park Drive, Suite 4, Branford, CT 06405, USA., Rotatori S; Enrich Biosystems Inc., 21 Business Park Drive, Suite 4, Branford, CT 06405, USA., Madden-Hennessey K; Enrich Biosystems Inc., 21 Business Park Drive, Suite 4, Branford, CT 06405, USA., Mohammed C; Enrich Biosystems Inc., 21 Business Park Drive, Suite 4, Branford, CT 06405, USA., Yang CH; Enrich Biosystems Inc., 21 Business Park Drive, Suite 4, Branford, CT 06405, USA., Urbani J; Enrich Biosystems Inc., 21 Business Park Drive, Suite 4, Branford, CT 06405, USA., Pettinelli J; Enrich Biosystems Inc., 21 Business Park Drive, Suite 4, Branford, CT 06405, USA., Liu X; Enrich Biosystems Inc., 21 Business Park Drive, Suite 4, Branford, CT 06405, USA., Zhao Q; Enrich Biosystems Inc., 21 Business Park Drive, Suite 4, Branford, CT 06405, USA. |
| Abstrakt: |
Enriching target cell clones from diverse cell populations is vital for many life science applications. We have developed a novel method to rapidly and efficiently purify specific clonal cell populations from a larger, heterogeneous group using the Enrich TroVo system (Enrich Biosystems Inc., CT, USA). This system takes advantage of microfabrication and optical technologies by utilizing small hydrogel wells to separate desired cell populations and an innovative patching technique to selectively eliminate undesired cells. This method allows the isolation and growth of desired cells with minimal impact on their viability and proliferation. We successfully isolated and expanded clonal cell populations of desired cells using two model cells. Compared with fluorescence-activated cell sorting, Enrich TroVo system offers a promising alternative for isolating of sensitive, adherent cells, that is, patient-derived cells. |
