Histones direct site-specific CRISPR spacer acquisition in model archaeon.

Autor: Watts EA; University of Georgia, Athens, GA, USA., Garrett SC; UConn Health, Farmington, CT, USA., Catchpole RJ; University of Georgia, Athens, GA, USA., Clark LM; University of Georgia, Athens, GA, USA., Sanders TJ; Colorado State University, Fort Collins, CO, USA., Marshall CJ; Colorado State University, Fort Collins, CO, USA., Wenck BR; Colorado State University, Fort Collins, CO, USA., Vickerman RL; Colorado State University, Fort Collins, CO, USA., Santangelo TJ; Colorado State University, Fort Collins, CO, USA., Fuchs R; New England Biolabs, Ipswich, MA, USA., Robb B; New England Biolabs, Ipswich, MA, USA., Olson S; UConn Health, Farmington, CT, USA., Graveley BR; UConn Health, Farmington, CT, USA. graveley@uchc.edu., Terns MP; University of Georgia, Athens, GA, USA. mterns@uga.edu.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2023 Sep; Vol. 8 (9), pp. 1682-1694. Date of Electronic Publication: 2023 Aug 07.
DOI: 10.1038/s41564-023-01446-3
Abstrakt: CRISPR-Cas systems provide heritable immunity against viruses and other mobile genetic elements by incorporating fragments of invader DNA into the host CRISPR array as spacers. Integration of new spacers is localized to the 5' end of the array, and in certain Gram-negative Bacteria this polarized localization is accomplished by the integration host factor. For most other Bacteria and Archaea, the mechanism for 5' end localization is unknown. Here we show that archaeal histones play a key role in directing integration of CRISPR spacers. In Pyrococcus furiosus, deletion of either histone A or B impairs integration. In vitro, purified histones are sufficient to direct integration to the 5' end of the CRISPR array. Archaeal histone tetramers and bacterial integration host factor induce similar U-turn bends in bound DNA. These findings indicate a co-evolution of CRISPR arrays with chromosomal DNA binding proteins and a widespread role for binding and bending of DNA to facilitate accurate spacer integration.
(© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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