Uropathogenic Escherichia coli wield enterobactin-derived catabolites as siderophores.

Autor:	Zou Z; Center for Women's Infectious Diseases Research, Washington University School of Medicine, St. Louis, Missouri, USA.; Department of Internal Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA., Robinson JI; Center for Women's Infectious Diseases Research, Washington University School of Medicine, St. Louis, Missouri, USA.; Department of Internal Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA., Steinberg LK; Center for Women's Infectious Diseases Research, Washington University School of Medicine, St. Louis, Missouri, USA.; Department of Internal Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA., Henderson JP; Center for Women's Infectious Diseases Research, Washington University School of Medicine, St. Louis, Missouri, USA.; Department of Internal Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, Missouri, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Jul 25. Date of Electronic Publication: 2023 Jul 25.
DOI:	10.1101/2023.07.25.550588
Abstrakt:	Uropathogenic E. coli (UPEC) secrete multiple siderophore types to scavenge extracellular iron(III) ions during clinical urinary tract infections, despite the metabolic costs of biosynthesis. Here we find the siderophore enterobactin and its related products to be prominent components of the iron-responsive extracellular metabolome of a model UPEC strain. Using defined enterobactin biosynthesis and import mutants, we identify lower molecular weight, dimeric exometabolites as products of incomplete siderophore catabolism, rather than prematurely released biosynthetic intermediates. In E. coli, iron acquisition from iron(III)-enterobactin complexes requires intracellular esterases that hydrolyze the siderophore. Although UPEC are equipped to consume the products of completely hydrolyzed enterobactin, we find that enterobactin and its derivatives may be incompletely hydrolyzed to yield products with retained siderophore activity. These results are consistent with catabolic inefficiency as means to obtain more than one iron ion per siderophore molecule. This is compatible with an evolved UPEC strategy to maximize the nutritional returns from metabolic investments in siderophore biosynthesis. Competing Interests: COMPETING INTERESTS The authors declare no competing interests.
Databáze:	MEDLINE
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