Gas vesicle-blood interactions enhance ultrasound imaging contrast.

Autor:	Ling B; Division of Chemistry and Chemical Engineering, California Institute of Technology; Pasadena, CA 91125, USA.; These authors contributed equally to this work., Ko JH; Division of Chemistry and Chemical Engineering, California Institute of Technology; Pasadena, CA 91125, USA.; These authors contributed equally to this work., Stordy B; Institute of Biomedical Engineering, University of Toronto; Toronto, ON M5S 3G9, Canada.; Terence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto; Toronto, ON M5S 3E1, Canada., Zhang Y; Institute of Biomedical Engineering, University of Toronto; Toronto, ON M5S 3G9, Canada.; Terence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto; Toronto, ON M5S 3E1, Canada.; Department of Chemistry, University of Toronto; Toronto, ON M5S 3H6, Canada., Didden TF; Division of Chemistry and Chemical Engineering, California Institute of Technology; Pasadena, CA 91125, USA., Malounda D; Division of Chemistry and Chemical Engineering, California Institute of Technology; Pasadena, CA 91125, USA., Swift MB; Division of Chemistry and Chemical Engineering, California Institute of Technology; Pasadena, CA 91125, USA., Chan WCW; Institute of Biomedical Engineering, University of Toronto; Toronto, ON M5S 3G9, Canada.; Terence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto; Toronto, ON M5S 3E1, Canada.; Department of Chemistry, University of Toronto; Toronto, ON M5S 3H6, Canada., Shapiro MG; Division of Chemistry and Chemical Engineering, California Institute of Technology; Pasadena, CA 91125, USA.; Division of Engineering and Applied Science, California Institute of Technology; Pasadena, CA 91125, USA.; Howard Hughes Medical Institute; Pasadena, CA 91125, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Jul 25. Date of Electronic Publication: 2023 Jul 25.
DOI:	10.1101/2023.07.24.550434
Abstrakt:	Gas vesicles (GVs) are genetically encoded, air-filled protein nanostructures of broad interest for biomedical research and clinical applications, acting as imaging and therapeutic agents for ultrasound, magnetic resonance, and optical techniques. However, the biomedical applications of GVs as a systemically injectable nanomaterial have been hindered by a lack of understanding of GVs' interactions with blood components, which can significantly impact in vivo performance. Here, we investigate the dynamics of GVs in the bloodstream using a combination of ultrasound and optical imaging, surface functionalization, flow cytometry, and mass spectrometry. We find that erythrocytes and serum proteins bind to GVs and shape their acoustic response, circulation time, and immunogenicity. We show that by modifying the GV surface, we can alter these interactions and thereby modify GVs' in vivo performance. These results provide critical insights for the development of GVs as agents for nanomedicine. Competing Interests: COMPETING INTERESTS The authors declare no competing financial interests.
Databáze:	MEDLINE
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