Genetic diversity of Plasmodium falciparum among asymptomatic pregnant women on intermittent preventive treatment with sulfadoxine-pyrimethamine in Nigeria.

Autor:	Olowe RA; Ladoke Akintola University of Technology, Department of Medical Microbiology and Parasitology., Ojo JA; Ladoke Akintola University of Technology, Department of Medical Microbiology and Parasitology., Funwei RI; Babcock University, Department of Pharmacology., Oyedeji SI; Federal University Oye-Ekiti, Department of Animal & Environmental Biology., Olowe OA; Ladoke Akintola University of Technology, Department of Medical Microbiology and Parasitology., Thomas BN; Rochester Institute of Technology, Department of Biomedical Sciences., Ojurongbe O; Ladoke Akintola University of Technology, Department of Medical Microbiology and Parasitology.
Jazyk:	angličtina
Zdroj:	African health sciences [Afr Health Sci] 2023 Mar; Vol. 23 (1), pp. 765-773.
DOI:	10.4314/ahs.v23i1.80
Abstrakt:	This study investigated the genetic diversity of Plasmodium falciparum among asymptomatic pregnant women on intermittent preventive treatment with sulfadoxine-pyrimethamine (IPTp-Sp) in Osogbo, southwest Nigeria. Blood sample was obtained from consenting pregnant women attending antenatal clinics. Microscopy and Polymerase chain reaction (PCR) were employed to diagnose and analyse genetic diversity. Of the 301 samples, 53 (18%) and 83 (28%) were positive for P. falciparum by microscopy and PCR, respectively. Using the merozoite surface protein ( msp)-1, msp-2 , and glutamate-rich protein (glurp) genes of P. falciparum as polymorphic markers, the msp-1 gene showed nine alleles with R033 (66.7%) being predominant, followed by K1 (45.5%) and MAD20 (33.3%). The msp-2 gene had 16 alleles (eight each for FC27 and 3D7). The 3D7 alleles (82.1%) was significantly more than FC27 alleles (48.2%) (p = 0.0093). Nine alleles were detected with glurp gene, presenting with the highest monoclonal and the lowest polyclonal infection. The multiplicity of infection (MOI) of 1.5, 1.8, and 1.2 were obtained for msp-1 , msp-2 and glurp genes. In light of the high P. falciparum genetic diversity among pregnant women on IPT-Sp in this study, additional strategies for preventing and controlling malaria in pregnancy might be required. (© 2023 Olowe RA et al.)
Databáze:	MEDLINE
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