Detection of Escherichia coli and other Enterobacteriales members in seabirds sampled along the Brazilian coast.

Autor: Cardoso MD; Programa de Pós-Graduação em Saúde Pública e Meio Ambiente, Escola Nacional de Saúde Pública Sérgio Arouca, Fundação Oswaldo Cruz, Rua Leopoldo Bulhões, 1480, Manguinhos, Rio de Janeiro 21041-210, RJ, Brazil. Electronic address: mairadc@gmail.com., Gonçalves VD; Laboratório de Referência Nacional de Enteroinfecções Bacterianas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Avenida Brasil, 4365, Pavilhão Rocha Lima, sala 316, Manguinhos, Rio de Janeiro 21040-360, RJ, Brazil. Electronic address: vegonc33@gmail.com., Grael AS; Setor de Animais Selvagens, Hospital Universitário de Medicina Veterinária Firmino Mársico Filho, Faculdade de Veterinária, Universidade Federal Fluminense, Avenida Almirante Ary Parreiras, 503, Vital Brazil, Niterói 24220-000, RJ, Brazil. Electronic address: andreagrael@uol.com.br., Pedroso VM; Centro de Recuperação de Animais Marinhos, Universidade Federal do Rio Grande, Rua Tenente Capitão Heitor Perdigão, 10, Centro, Rio Grande 96200-580, RS, Brazil. Electronic address: vanessacramfurg@gmail.com., Pires JR; Centro de Recuperação de Fauna Silvestre, Universidade Estácio de Sá - Estrada da Boca do Mato, 850, Vargem Pequena, Rio de Janeiro 22783-320, RJ, Brazil. Electronic address: jefveterinario@yahoo.com.br., Travassos CEPF; Laboratório de Sanidade Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego, 2000, Horto, Campos dos Goytacazes, Rio de Janeiro 28013-602, Brazil. Electronic address: cacaetravassos@gmail.com., Domit C; Laboratório de Ecologia e Conservação, Centro de Estudos do Mar, Universidade Federal do Paraná, Paraná CEP 83255-000, Brazil. Electronic address: cadomit@gmail.com., Vieira-Da-Motta O; Laboratório de Sanidade Animal, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego, 2000, Horto, Campos dos Goytacazes, Rio de Janeiro 28013-602, Brazil. Electronic address: olney@uenf.br., Dos Prazeres Rodrigues D; Laboratório de Referência Nacional de Enteroinfecções Bacterianas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Avenida Brasil, 4365, Pavilhão Rocha Lima, sala 316, Manguinhos, Rio de Janeiro 21040-360, RJ, Brazil. Electronic address: daliarodrigues@yahoo.com.br., Siciliano S; Departamento de Ciências Biológicas, Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz, Rua Leopoldo Bulhões, 1.480, Manguinhos, Rio de Janeiro 21041-910, RJ, Brazil. Electronic address: gemmlagos@gmail.com.
Jazyk: angličtina
Zdroj: Preventive veterinary medicine [Prev Vet Med] 2023 Sep; Vol. 218, pp. 105978. Date of Electronic Publication: 2023 Jul 26.
DOI: 10.1016/j.prevetmed.2023.105978
Abstrakt: Escherichia coli, an Enterobacterales member, is a normal representative of the microbiota of homeothermic animals. Most strains are commensal, but several pathotypes can cause disease, and numerous antimicrobial resistance factors have been identified. These bacteria have spread rapidly in recent years, highlighting the importance of screening the environment and non-human reservoirs for virulent strains and/or those presenting resistance factors, in addition to other microorganisms of public health importance. In this context, this study aimed to survey Enterobacteriales present in seabirds sampled along the Brazilian coast, comparing findings between migratory and resident birds, as well as between wrecked and non-wrecked animals. Escherichia coli pathotypes were also characterized through rapid seroagglutination and polymerase chain reaction techniques and antimicrobial resistance profiles were investigated through the disc agar diffusion method. Cloacal, ocular, oral, tracheal, and skin lesion swabs, as well as fresh feces, were collected from 122 seabirds. The findings indicate these animals as important hosts for opportunistic human pathogens. Escherichia coli strains were identified in 70 % of the analyzed seabirds, 62 % of which displaying resistant or intermediate profiles to at least one antimicrobial, while 7% were multiresistant. Resistance to tetracycline (22 %), nalidixic acid (15 %), trimethoprim-sulfamethozaxol (14 %) and ampicillin (12 %) were the most prevalent. Resistance to cefoxitin, a critically important antimicrobial for human medicine, was also detected. Virulence genes for one of the EAEC, ETEC or EPEC pathotypes were detected in 30 % of the identified strains, the first two described in seabirds for the first time. The EAEC gene was detected in 25 % of the sampled seabirds, all resident, 8 % of which exhibited a multidrug-resistant profile. Thus, seabirds comprise important reservoirs for this pathotype. Escherichia coli was proven an ubiquitous and well-distributed bacterium, present in all evaluated bird species and sampling sites (except Marajó Island). According to the chi-square test, no significant differences between E. coli prevalences or antimicrobial resistance profiles between migratory and resident and between wrecked and non-wrecked seabirds were observed. Thus, migratory birds do not seem to contribute significantly to E. coli frequencies, pathotypes or antimicrobial resistance rates on the Brazilian coast.
Competing Interests: Declaration of Competing Interest We have no conflicts of interest to disclose.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: