Altered Expression of hsa_circ_0001445 and hsa_circ_0020397 in Breast Cancer Representing Associations with BMI and Reproductive Factors.

Autor: Firoozi Z; Department of Medical Genetics, Fasa University of Medical Sciences, Fasa, Iran., Mohammadisoleimani E; Department of Medical Biotechnology, Fasa University of Medical Sciences, Fasa, Iran., Dastsooz H; IIGM-Italian Institute for Genomic Medicine, c/o IRCCS, Candiolo, Torino, Italy.; Candiolo Cancer Institute, FPO-IRCCS, Candiolo Cancer (IT), Torino, Italy.; Department of Life Sciences and Systems Biology, University of Turin, Via Accademia Albertina, Turin, Italy., Daraei A; Department of Medical Genetics, School of Medicine, Babol University of Medical Sciences, Babol, Iran., Dastgheib SA; Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Raoofat A; Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Mansoori H; Department of Medical Genetics, Fasa University of Medical Sciences, Fasa, Iran., Mansoori Y; Department of Medical Genetics, Fasa University of Medical Sciences, Fasa, Iran.; Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran., Naghizadeh MM; Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran.
Jazyk: angličtina
Zdroj: Archives of Iranian medicine [Arch Iran Med] 2022 Dec 01; Vol. 25 (12), pp. 817-827. Date of Electronic Publication: 2022 Dec 01.
DOI: 10.34172/aim.2022.127
Abstrakt: Background: Circular RNAs (circRNAs), one of the recent subclasses of non-coding RNAs (ncRNAs), show pivotal functions in regulation of gene expression and have significant roles in malignancies including breast cancer (BC). This study was aimed to assess the hsa_circ_0001445 and hsa_circ_0020397 expression and role in BC, as well as the potential circRNA/miRNA/mRNA crosstalk in these contexts.
Methods: The expression of hsa_circ_0001445 and hsa_circ_0020397 in 50 breast tumors and 50 normal tissues adjacent to the tumors was investigated using quantitative real-time polymerase chain reaction (qRT-PCR). Finally, bioinformatics analyses were used to uncover hsa_circ_0001445, hsa_circ_0020397-miRNA-mRNA potential regulatory networks.
Results: The hsa_circ_0001445 expression was considerably downregulated in malignant tissues compared to their normal counterparts ( P =0.020), while the hsa_circ_0020397 showed an upregulated pattern ( P <0.001). Additionally, it was observed that the higher expression of hsa_circ_0001445 was associated with hair dye avoidance ( P =0.034) and normal body mass index (BMI) ( P =0.016) while hsa_circ_0020397 over-expression had an important association with a lack of vitamin D consumption ( P =0.039). On the other hand, lower expression of hsa_circ_0001445 was significantly associated with age at menarche ˂14 years ( P =0.027). Our study also revealed that the two circRNAs have potential ability to regulate key mRNAs and miRNAs in competing endogenous RNA (ceRNA) networks.
Conclusion: It is suggested that hsa_circ_0001445 and hsa_circ_0020397 with two opposite roles may be involved in BC development through sponging some miRNAs regulating ceRNA networks. However, their molecular interactions should be validated by further functional studies.
(© 2022 The Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.)
Databáze: MEDLINE
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