Laser texturing of additively manufactured implants: A tool to programme biological response.

Autor: Villapún VM; School of Chemical Engineering, University of Birmingham, Edgbaston B15 2TT, United Kingdom. Electronic address: v.m.villapun@bham.ac.uk., Man K; Department of Oral and Maxillofacial Surgery & Special Dental Care, University Medical Center, Utrecht GA 3508, the Netherlands., Carter L; School of Chemical Engineering, University of Birmingham, Edgbaston B15 2TT, United Kingdom., Penchev P; Department of Mechanical Engineering, University of Birmingham, Edgbaston B15 2TT, United Kingdom., Dimov S; Department of Mechanical Engineering, University of Birmingham, Edgbaston B15 2TT, United Kingdom., Cox S; School of Chemical Engineering, University of Birmingham, Edgbaston B15 2TT, United Kingdom. Electronic address: s.c.cox@bham.ac.uk.
Jazyk: angličtina
Zdroj: Biomaterials advances [Biomater Adv] 2023 Oct; Vol. 153, pp. 213574. Date of Electronic Publication: 2023 Jul 29.
DOI: 10.1016/j.bioadv.2023.213574
Abstrakt: The advent of additive manufacturing (AM) is rapidly shaping healthcare technologies pushing forward personalisation and enhanced implant functionalisation to improve clinical outcomes. AM techniques such as powder bed fusion (PBF) have been adopted despite the need to modify the as-built surface post manufacture. Medical device manufacturers have focused their efforts on refining various physical and chemical surface finishing approaches, however there is little consensus and some methods risk geometry alteration or contamination. This has led to a growing interest in laser texturing technologies to engineer the device surface. Herein, several bioinspired micro and nano textures were applied to laser PBF Ti-6Al-V4 substrates to alter physicochemical properties and in-turn we sought to understand what influences these alterations had on a human osteosarcoma cell line (MG63). Significant variations in roughness and time dependent contact angles were revealed between different patterns provide a tool to elicit desired biological responses. All surface treatments effectively enhanced early cell behaviour and in particular coverage was increased for the micro-textures. Influence of the patterns on cell differentiation was less consistent with alkaline phosphatase content increased only for the channel, grid and dual textures. While long term (21 days) mineralisation was found to be significantly enhanced in grids, dual, triangles and shark skin textures. Further regression analysis of all physicochemical and biological variables indicated that several properties should be used to strongly correlate cell behaviour, resulting in 82 % of the 21 day mineralisation dataset explained through a combination of roughness kurtosis and glycerol contact angle. Overall, this manuscript demonstrates the ability of laser texturing to offer tailored cell-surface interactions, which can be tuned to offer a tool to drive functional customisation of anatomically customised medical devices.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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