Genetic rearrangements in Pseudomonas amygdali pathovar aesculi shape coronatine plasmids.

Autor: Nielsen TK; Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark., Winther-Have CS; Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark; Globe Institute, University of Copenhagen, 1350 Copenhagen, Denmark., Thomsen IM; Department of Geosciences and Natural Resource Management, University of Copenhagen, 1958 Frederiksberg C, Denmark., Jackson RW; School of Biosciences and the Birmingham Institute of Forest Research, University of Birmingham, Birmingham B15 2TT, United Kingdom., Rabiey M; School of Biosciences and the Birmingham Institute of Forest Research, University of Birmingham, Birmingham B15 2TT, United Kingdom., Hennessy RC; Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark., Bak F; Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark., Kot W; Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark., Nicolaisen MH; Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark., Carstens AB; Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark., Hansen LH; Department of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, Denmark. Electronic address: lhha@plen.ku.dk.
Jazyk: angličtina
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2023 Sep; Vol. 113, pp. 105486. Date of Electronic Publication: 2023 Aug 02.
DOI: 10.1016/j.meegid.2023.105486
Abstrakt: Plant pathogenic Pseudomonas species use multiple classes of toxins and virulence factors during host infection. The genes encoding these pathogenicity factors are often located on plasmids and other mobile genetic elements, suggesting that they are acquired through horizontal gene transfer to confer an evolutionary advantage for successful adaptation to host infection. However, the genetic rearrangements that have led to mobilization of the pathogenicity genes are not fully understood. In this study, we have sequenced and analyzed the complete genome sequences of four Pseudomonas amygdali pv. aesculi (Pae), which infect European horse chestnut trees (Aesculus hippocastanum) and belong to phylogroup 3 of the P. syringae species complex. The four investigated genomes contain six groups of plasmids that all encode pathogenicity factors. Effector genes were found to be mostly associated with insertion sequence elements, suggesting that virulence genes are generally mobilized and potentially undergo horizontal gene transfer after transfer to a conjugative plasmid. We show that the biosynthetic gene cluster encoding the phytotoxin coronatine was recently transferred from a chromosomal location to a mobilizable plasmid that subsequently formed a co-integrate with a conjugative plasmid.
Competing Interests: Declaration of Competing Interest The authors declare no competing interests.
(Crown Copyright © 2023. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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