Repetitive mRNA vaccination is required to improve the quality of broad-spectrum anti-SARS-CoV-2 antibodies in the absence of CXCL13.

Autor:	Azarias Da Silva M; INSERM-U1124, Université Paris Cité, Paris, France., Nioche P; INSERM-U1124, Université Paris Cité, Paris, France.; Structural and Molecular Analysis Platform, BioMedTech Facilities INSERM US36-CNRS UMS2009, Université Paris Cité, Paris, France., Soudaramourty C; INSERM-U1124, Université Paris Cité, Paris, France., Bull-Maurer A; Université de Tours, INSERM, UMR1259 MAVIVH, Tours, France., Tiouajni M; INSERM-U1124, Université Paris Cité, Paris, France.; Structural and Molecular Analysis Platform, BioMedTech Facilities INSERM US36-CNRS UMS2009, Université Paris Cité, Paris, France., Kong D; Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Japan.; Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan., Zghidi-Abouzid O; CHU de Québec-Université Laval Research Center, Québec City, Québec, Canada., Picard M; INSERM-U1124, Université Paris Cité, Paris, France., Mendes-Frias A; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal., Santa-Cruz A; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.; Department of Internal Medicine, Hospital of Braga, Braga, Portugal., Carvalho A; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.; Department of Internal Medicine, Hospital of Braga, Braga, Portugal., Capela C; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.; Department of Internal Medicine, Hospital of Braga, Braga, Portugal., Pedrosa J; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal., Castro AG; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal., Loubet P; Service des Maladies Infectieuses et Tropicales, CHU de Nîmes, Nîmes, France., Sotto A; Service des Maladies Infectieuses et Tropicales, CHU de Nîmes, Nîmes, France., Muller L; Service de Réanimation Chirugicale, CHU de Nîmes, Nîmes, France., Lefrant JY; Service de Réanimation Chirugicale, CHU de Nîmes, Nîmes, France., Roger C; Service de Réanimation Chirugicale, CHU de Nîmes, Nîmes, France., Claret PG; Urgences Médico-Chirugicales Hospitalisation, CHU de Nîmes, Nîmes, France., Duvnjak S; Service de Gérontologie et Prévention du Vieillissement, CHU de Nîmes, Nîmes, France., Tran TA; Service de Pédiatrie, CHU de Nîmes, Nîmes, France., Tokunaga K; Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan., Silvestre R; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal., Corbeau P; Institut de Génétique Humaine, UMR9002 CNRS-Université de Montpellier, Montpellier, France.; Laboratoire d'Immunologie, CHU de Nîmes, Nîmes, France., Mammano F; INSERM-U1124, Université Paris Cité, Paris, France.; Université de Tours, INSERM, UMR1259 MAVIVH, Tours, France., Estaquier J; INSERM-U1124, Université Paris Cité, Paris, France.; CHU de Québec-Université Laval Research Center, Québec City, Québec, Canada.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2023 Aug 04; Vol. 9 (31), pp. eadg2122. Date of Electronic Publication: 2023 Aug 04.
DOI:	10.1126/sciadv.adg2122
Abstrakt:	Since the initial spread of severe acute respiratory syndrome coronavirus 2 infection, several viral variants have emerged and represent a major challenge for immune control, particularly in the context of vaccination. We evaluated the quantity, quality, and persistence of immunoglobulin G (IgG) and IgA in individuals who received two or three doses of messenger RNA (mRNA) vaccines, compared with previously infected vaccinated individuals. We show that three doses of mRNA vaccine were required to match the humoral responses of preinfected vaccinees. Given the importance of antibody-dependent cell-mediated immunity against viral infections, we also measured the capacity of IgG to recognize spike variants expressed on the cell surface and found that cross-reactivity was also strongly improved by repeated vaccination. Last, we report low levels of CXCL13, a surrogate marker of germinal center activation and formation, in vaccinees both after two and three doses compared with preinfected individuals, providing a potential explanation for the short duration and low quality of Ig induced.
Databáze:	MEDLINE
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