Effects of Capsular Polysaccharide amount on Pneumococcal-Host interactions.

Autor: Zhu J; Department of Microbiology, New York University Grossman School of Medicine, New York, New York, United States of America., Abruzzo AR; Department of Microbiology, New York University Grossman School of Medicine, New York, New York, United States of America., Wu C; Department of Microbiology, New York University Grossman School of Medicine, New York, New York, United States of America., Bee GCW; Department of Microbiology, New York University Grossman School of Medicine, New York, New York, United States of America., Pironti A; Department of Microbiology, New York University Grossman School of Medicine, New York, New York, United States of America.; Microbial Computational Genomic Core Lab, Department of Microbiology, New York University Grossman School of Medicine, New York, New York, United States of America., Putzel G; Department of Microbiology, New York University Grossman School of Medicine, New York, New York, United States of America.; Microbial Computational Genomic Core Lab, Department of Microbiology, New York University Grossman School of Medicine, New York, New York, United States of America., Aggarwal SD; Department of Microbiology, New York University Grossman School of Medicine, New York, New York, United States of America., Eichner H; Department of Microbiology, New York University Grossman School of Medicine, New York, New York, United States of America.; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, and Clinical Microbiology, Bioclinicum, Karolinska University Hospital Solna, Solna, Sweden., Weiser JN; Department of Microbiology, New York University Grossman School of Medicine, New York, New York, United States of America.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2023 Aug 04; Vol. 19 (8), pp. e1011509. Date of Electronic Publication: 2023 Aug 04 (Print Publication: 2023).
DOI: 10.1371/journal.ppat.1011509
Abstrakt: Among the many oral streptococci, Streptococcus pneumoniae (Spn) stands out for the capacity of encapsulated strains to cause invasive infection. Spread beyond upper airways, however, is a biological dead end for the organism, raising the question of the benefits of expending energy to coat its surface in a thick layer of capsular polysaccharide (CPS). In this study, we compare mutants of two serotypes expressing different amounts of CPS and test these in murine models of colonization, invasion infection and transmission. Our analysis of the effect of CPS amount shows that Spn expresses a capsule of sufficient thickness to shield its surface from the deposition of complement and binding of antibody to underlying epitopes. While effective shielding is permissive for invasive infection, its primary contribution to the organism appears to be in the dynamics of colonization. A thicker capsule increases bacterial retention in the nasopharynx, the first event in colonization, and also impedes IL-17-dependent clearance during late colonization. Enhanced colonization is associated with increased opportunity for host-to-host transmission. Additionally, we document substantial differences in CPS amount among clinical isolates of three common serotypes. Together, our findings show that CPS amount is highly variable among Spn and could be an independent determinant affecting host interactions.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Zhu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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