Expression and Functional Studies of Leaf, Floral, and Fruit Developmental Genes in Non-model Species.
| Autor: | Pabón-Mora N; Instituto de Biología, Universidad de Antioquia, Medellín, Colombia. lucia.pabon@udea.edu.co., Suárez-Baron H; Instituto de Biología, Universidad de Antioquia, Medellín, Colombia.; Departamento de Ciencias Naturales y Matemáticas, Pontificia Universidad Javeriana, Cali, Colombia., Madrigal Y; Instituto de Biología, Universidad de Antioquia, Medellín, Colombia., Alzate JF; Centro Nacional de Secuenciación Genómica-CNSG, Sede de Investigación Universitaria-SIU, Medellín, Antioquia, Colombia., González F; Instituto de Ciencias Naturales, Universidad Nacional de Colombia, Sede Bogotá, Bogotá, Colombia. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2686, pp. 365-401. |
| DOI: | 10.1007/978-1-0716-3299-4_19 |
| Abstrakt: | Researchers working on evolutionary developmental plant biology are inclined to choose non-model taxa to address how specific features have been acquired during ontogeny and fixed during phylogeny. In this chapter we describe methods to extract RNA, to assemble de-novo transcriptomes, to isolate orthologous genes within gene families, and to evaluate expression and function of target genes. We have successfully optimized these protocols for non-model plant species including ferns, gymnosperms, and a large assortment of angiosperms. In the latter, we have ranged a large number of families including Aristolochiaceae, Apodanthaceae, Chloranthaceae, Orchidaceae, Papaveraceae, Rubiaceae, Solanaceae, and Tropaeolaceae. (© 2023. Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|