Synergistic effect of hydralazine associated with triazoles on Candida spp. in planktonic cells.

Autor:	do Nascimento FB; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, Ceará, 60430-160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil., Valente Sá LG; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, Ceará, 60430-160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil.; Christus University Center (UNICHRISTUS), Fortaleza, Ceará, 60190-180, Brazil., de Andrade Neto JB; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, Ceará, 60430-160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil.; Christus University Center (UNICHRISTUS), Fortaleza, Ceará, 60190-180, Brazil., Sampaio LS; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, Ceará, 60430-160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil., Queiroz HA; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, Ceará, 60430-160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil., Silva LJ; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, Ceará, 60430-160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil., Cabral VP; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, Ceará, 60430-160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil., Rodrigues DS; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, Ceará, 60430-160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil., Pereira SC; Hospital Dr. Carlos Alberto Studart, Fortaleza, Ceará, 60840-285, Brazil., Cavalcanti BC; Drug Research & Development Center, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil.; Department of Physiology & Pharmacology, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil., Silva J; Department of Chemistry, Group of Theoretical Chemistry & Electrochemistry (GQTE), State University of Ceará, Limoeiro do Norte, Ceará, 62930-000, Brazil., Marinho ES; Department of Chemistry, Group of Theoretical Chemistry & Electrochemistry (GQTE), State University of Ceará, Limoeiro do Norte, Ceará, 62930-000, Brazil., Santos HS; Department of Chemistry, Group of Theoretical Chemistry & Electrochemistry (GQTE), State University of Ceará, Limoeiro do Norte, Ceará, 62930-000, Brazil., Moraes MO; Drug Research & Development Center, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil.; Department of Physiology & Pharmacology, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil., Nobre Júnior HV; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, Ceará, 60430-160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil., Silva CR; School of Pharmacy, Laboratory of Bioprospection of Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, Ceará, 60430-160, Brazil.; Drug Research & Development Center, Federal University of Ceará, Fortaleza, Ceará, 60430-275, Brazil.
Jazyk:	angličtina
Zdroj:	Future microbiology [Future Microbiol] 2023 Jul; Vol. 18, pp. 661-672. Date of Electronic Publication: 2023 Aug 04.
DOI:	10.2217/fmb-2023-0012
Abstrakt:	Objective: To evaluate the antifungal activity of hydralazine hydrochloride alone and in synergy with azoles against Candida spp. and the action mechanism. Methods: We used broth microdilution assays to determine the MIC, checkerboard assays to investigate synergism, and flow cytometry and molecular docking tests to ascertain action mechanism. Results: Hydralazine alone had antifungal activity in the range of 16-128 μg/ml and synergistic effect with itraconazole versus 100% of the fungal isolates, while there was synergy with fluconazole against 11.11% of the isolates. There was molecular interaction with the receptors exo-B(1,3)-glucanase and CYP51, causing reduced cell viability and DNA damage. Conclusion: Hydralazine is synergistic with itraconazole and triggers cell death of Candida spp. at low concentrations, demonstrating antifungal potential.
Databáze:	MEDLINE
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