Nanopore sequencing technology for clinical diagnosis of infectious diseases where laboratory capacity is meager: A case report.

Autor:	Kumburu HH; Kilimanjaro Christian Medical Centre (KCMC), Tanzania.; Kilimanjaro Clinical Research Institute (KCRI), Sokoine Road, P. O. Box. 2236, Moshi, Tanzania.; Kilimanjaro Christian Medical University College (KCMUCo), 2240 Moshi Kilimanajro, Tanzania., Shayo M; Kilimanjaro Christian Medical University College (KCMUCo), 2240 Moshi Kilimanajro, Tanzania., van Zwetslaar M; Kilimanjaro Clinical Research Institute (KCRI), Sokoine Road, P. O. Box. 2236, Moshi, Tanzania., Njau J; Kilimanjaro Clinical Research Institute (KCRI), Sokoine Road, P. O. Box. 2236, Moshi, Tanzania., Kuchaka DJ; Kilimanjaro Clinical Research Institute (KCRI), Sokoine Road, P. O. Box. 2236, Moshi, Tanzania.; The Nelson Mandela African Institution of Science and Technology (NM-AIST), Tanzania., Ignas IP; Kilimanjaro Clinical Research Institute (KCRI), Sokoine Road, P. O. Box. 2236, Moshi, Tanzania., Wadugu B; Kilimanjaro Clinical Research Institute (KCRI), Sokoine Road, P. O. Box. 2236, Moshi, Tanzania., Kasworm R; Machame Lutheran Hospital, Tanzania., Masaki LJ; Machame Lutheran Hospital, Tanzania., Hallgren MB; Technical University of Denmark (DTU), Denmark., Clausen PTLC; Technical University of Denmark (DTU), Denmark., Mmbaga BT; Kilimanjaro Christian Medical Centre (KCMC), Tanzania.; Kilimanjaro Clinical Research Institute (KCRI), Sokoine Road, P. O. Box. 2236, Moshi, Tanzania.; Kilimanjaro Christian Medical University College (KCMUCo), 2240 Moshi Kilimanajro, Tanzania., Aarestrup FM; Technical University of Denmark (DTU), Denmark., Sonda TB; Kilimanjaro Christian Medical Centre (KCMC), Tanzania.; Kilimanjaro Clinical Research Institute (KCRI), Sokoine Road, P. O. Box. 2236, Moshi, Tanzania.; Kilimanjaro Christian Medical University College (KCMUCo), 2240 Moshi Kilimanajro, Tanzania.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2023 Jun 28; Vol. 9 (7), pp. e17439. Date of Electronic Publication: 2023 Jun 28 (Print Publication: 2023).
DOI:	10.1016/j.heliyon.2023.e17439
Abstrakt:	In resource-limited settings, patients are often first presented to clinical settings when seriously ill and access to proper clinical microbial diagnostics is often very limited or non-existing. On February 16th , 2022 we were on a field trip to test a completely field-deployable metagenomics sequencing set-up, that includes DNA purification, sequencing, and bioinformatics analyses using bioinformatics tools installed on a laptop for water samples, just outside Moshi, Tanzania. On our way to the test site, we were contacted by the nearby Machame hospital regarding a child seriously ill with diarrhea and not responding to treatment. Within the same day, we conducted an onsite metagenomics examination of a fecal sample from the child, and Campylobacter jejuni was identified as the causative agent. The treatment was subsequently changed, with almost immediate improvement, and the child was discharged on February 21st. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 Published by Elsevier Ltd.)
Databáze:	MEDLINE
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