T-tubule recovery after detubulation in isolated mouse cardiomyocytes.

Autor: Tamkus G; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA., Uchida K; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA., Lopatin AN; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA.
Jazyk: angličtina
Zdroj: Physiological reports [Physiol Rep] 2023 Aug; Vol. 11 (15), pp. e15779.
DOI: 10.14814/phy2.15779
Abstrakt: Remodeling of cardiac t-tubules in normal and pathophysiological conditions is an important process contributing to the functional performance of the heart. While it is well documented that deterioration of t-tubule network associated with various pathological conditions can be reversed under certain conditions, the mechanistic understanding of the recovery process is essentially lacking. Accordingly, in this study we investigated some aspects of the recovery of t-tubules after experimentally-induced detubulation. T-tubules of isolated mouse ventricular myocytes were first sealed using osmotic shock approach, and their recovery under various experimental conditions was then characterized using electrophysiologic and imaging techniques. The data show that t-tubule recovery is a strongly temperature-dependent process involving reopening of previously collapsed t-tubular segments. T-tubule recovery is slowed by (1) metabolic inhibition of cells, (2) reducing influx of extracellular Ca 2+ as well as by (3) both stabilization and disruption of microtubules. Overall, the data show that t-tubule recovery is a highly dynamic process involving several central intracellular structures and processes and lay the basis for more detailed investigations in this area.
(© 2023 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje