Escherichia coli isolates from meat and abattoirs environment in Egypt: molecular characterization and control by nanosilver particles.

Autor: Mansour AM; Department of Animal Hygiene and Zoonosis, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt., Nossair MA; Department of Animal Hygiene and Zoonosis, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt., Soliman FS; Department of Animal Hygiene and Zoonosis, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt., Tawfik RG; Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt., Elekhnawy E; Pharmaceutical Microbiology Department, Faculty of Pharmacy, Tanta University, Tanta, Egypt., Al-Kuraishy HM; Department of Clinical Pharmacology and Medicine, College of Medicine, ALmustansiriyia University, M.B.ch.b, FRCP, Bagdad, Iraq., Batiha GE; Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt., Mahmoud MH; Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia., Alexiou A; Department of Science and Engineering, Novel Global Community Educational Foundation, Hebersham, Australia.; AFNP Med, Wien, Austria., Shawky MM; Department of Food Hygiene, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
Jazyk: angličtina
Zdroj: International journal of environmental health research [Int J Environ Health Res] 2024 Mar; Vol. 34 (3), pp. 1751-1762. Date of Electronic Publication: 2023 Aug 03.
DOI: 10.1080/09603123.2023.2243828
Abstrakt: Three hundred samples, including meat from the slaughtered carcass and water, air samples, and swabs from the floor, wall, and employees' hands, were collected from five municipal abattoirs spread across several Egyptian provinces. The Escherichia coli was isolated from floor swabs, meat, air, wall, hand, and water samples. Serotyping of the recovered isolates clarified the presence of various serotypes, including enterohemorrhagic serotypes (O 111 : H 4 , O 128 : H 2 , and O 127 : H 6 ) and enterotoxigenic serotypes (O 44 : H 18 and O 125 : H 21 ). The isolates were resistant to cefotaxime (100%), amoxiclav (80%), then rifampin (66.7%). The stx 1 gene, stx 2 gene, eaeA gene, bla CMY2 gene and iss gene were detected in 10-80 % of the isolates. Nanosilver (AgNPs) showed that 12.5 ppm was the lowest concentration that prevented bacterial growth. It was observed that 12% of workers wore a clean white coat, only 24% washed their hands between activities during work, only 14% used soap for hand washing, and 42% utilized the same knife for meat and its offal.
Databáze: MEDLINE