Restoration of energy homeostasis under oxidative stress: Duo synergistic AMPK pathways regulating arginine kinases.

Autor: Zhang N; College of Plant Protection, Yangzhou University, Yangzhou, China.; Jiangsu Lixiahe Institute of Agricultural Sciences, Yangzhou, China., Meng X; College of Plant Protection, Yangzhou University, Yangzhou, China., Jiang H; College of Plant Protection, Yangzhou University, Yangzhou, China., Ge H; College of Plant Protection, Yangzhou University, Yangzhou, China., Qian K; College of Plant Protection, Yangzhou University, Yangzhou, China., Zheng Y; College of Plant Protection, Yangzhou University, Yangzhou, China., Park Y; Department of Entomology, Kansas State University, Manhattan, Kansas, United States of America., Wang J; College of Plant Protection, Yangzhou University, Yangzhou, China.
Jazyk: angličtina
Zdroj: PLoS genetics [PLoS Genet] 2023 Aug 03; Vol. 19 (8), pp. e1010843. Date of Electronic Publication: 2023 Aug 03 (Print Publication: 2023).
DOI: 10.1371/journal.pgen.1010843
Abstrakt: Rapid depletion of cellular ATP can occur by oxidative stress induced by reactive oxygen species (ROS). Maintaining energy homeostasis requires the key molecular components AMP-activated protein kinase (AMPK) and arginine kinase (AK), an invertebrate orthologue of the mammalian creatine kinase (CK). Here, we deciphered two independent and synergistic pathways of AMPK acting on AK by using the beetle Tribolium castaneum as a model system. First, AMPK acts on transcriptional factor forkhead box O (FOXO) leading to phosphorylation and nuclear translocation of the FOXO. The phospho-FOXO directly promotes the expression of AK upon oxidative stress. Concomitantly, AMPK directly phosphorylates the AK to switch the direction of enzymatic catalysis for rapid production of ATP from the phosphoarginine-arginine pool. Further in vitro assays revealed that Sf9 cells expressing phospho-deficient AK mutants displayed the lower ATP/ADP ratio and cell viability under paraquat-induced oxidative stress conditions when compared with Sf9 cells expressing wild-type AKs. Additionally, the AMPK-FOXO-CK pathway is also involved in the restoration of ATP homeostasis under oxidative stress in mammalian HEK293 cells. Overall, we provide evidence that two distinct AMPK-AK pathways, transcriptional and post-translational regulations, are coherent responders to acute oxidative stresses and distinguished from classical AMPK-mediated long-term metabolic adaptations to energy challenge.
Competing Interests: The authors declare that they have no competing interests.
(Copyright: © 2023 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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