Genetic Analysis of LigA, LipL32, Loa22, and OmpL1 Antigens from Leptospira spp. Sejroe Serogroup: How Close We Are To a Vaccine Against Bovine Leptospirosis?

Autor: Di Azevedo MIN; Laboratory of Veterinary Bacteriology, Fluminense Federal University, Biomedical Institute, Niterói, Rio de Janeiro, Brazil. isabeldiazevedo@gmail.com., Borges ALDSB; Laboratory of Veterinary Bacteriology, Fluminense Federal University, Biomedical Institute, Niterói, Rio de Janeiro, Brazil., Kremer F; Technological Development Center, Federal University of Pelotas, Capão do Leão, Rio Grande do Sul, Brazil., de Melo JDSL; Laboratory of Veterinary Bacteriology, Fluminense Federal University, Biomedical Institute, Niterói, Rio de Janeiro, Brazil., Carvalho-Costa FA; Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil., Lilenbaum W; Laboratory of Veterinary Bacteriology, Fluminense Federal University, Biomedical Institute, Niterói, Rio de Janeiro, Brazil.
Jazyk: angličtina
Zdroj: Current microbiology [Curr Microbiol] 2023 Aug 03; Vol. 80 (9), pp. 310. Date of Electronic Publication: 2023 Aug 03.
DOI: 10.1007/s00284-023-03414-w
Abstrakt: Bovine leptospirosis has as main causative agents Leptospira spp. from Sejroe serogroup. Vaccination is a crucial step to control this infection. The use of conserved proteins among Leptospira spp. is of great importance for a protective immune response. The aim of the present study is to genetically analyze antigens of Leptospira spp. from Sejroe serogroup strains isolated from cattle for a preliminary evaluation of vaccine candidates. Genes associated with antigenicity-LigA, LipL32, Loa22, and OmpL1-were analyzed through bioinformatic and immunoinformatic tools. Despite high diversity observed in strains, on an amino acid level, highly conserved regions were observed (> 90%), particularly in LipL32 gene. Moreover, highly conserved amino acid regions (> 30 aa) were observed in all genes, regardless of species, geographical origin or biological source of isolation. Superposed structures of protein fragments including all the predicted MHC-II and B-Cell epitopes were demonstrated. Results presented herein are preliminary, but a fundamental step towards the development of an efficient vaccine against bovine leptospirosis, a silent but enormously concerning disease.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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