The initial steps toward the formation of somatic tissue banks and cell cultures derived from captive Antillean manatee (Trichechus manatus manatus) skin biopsies.

Autor: Nascimento MB; Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid (UFERSA), Mossoró, Rio Grande do Norte, Brazil., Moura YBF; Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid (UFERSA), Mossoró, Rio Grande do Norte, Brazil., Oliveira REM; Laboratory of Applied Animal Morphophysiology, UFERSA, Mossoró, Rio Grande do Norte, Brazil., Lira GPO; Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid (UFERSA), Mossoró, Rio Grande do Norte, Brazil., Borges AA; Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid (UFERSA), Mossoró, Rio Grande do Norte, Brazil., Luna FO; National Center for Research and Conservation of Aquatic Mammals by Chico Mendes Institute for Biodiversity Conservation, Santos, São Paulo, Brazil., Attademo FLN; National Center for Research and Conservation of Aquatic Mammals by Chico Mendes Institute for Biodiversity Conservation, Santos, São Paulo, Brazil.; Laboratory of Behavior and Conservation Ecology, Department of Zoology, Recife, Pernambuco, Brazil., Pereira AF; Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid (UFERSA), Mossoró, Rio Grande do Norte, Brazil.
Jazyk: angličtina
Zdroj: Zoo biology [Zoo Biol] 2023 Nov-Dec; Vol. 42 (6), pp. 709-722. Date of Electronic Publication: 2023 Aug 03.
DOI: 10.1002/zoo.21798
Abstrakt: The declining population of the Antillean manatee caused by ecosystem degradation and rising pollution has prompted interest in developing conservation strategies for this species. Given this scenario, somatic tissue banks are important tools for acquiring knowledge about the species, as well as for obtaining somatic cells for biotechnological and ecotoxicological applications. Therefore, we aimed to assess the effects of slow freezing (SF) and solid-surface vitrification (SSV) of the dermis of captive Antillean manatees on the histology and ultrastructure of the tissue and cell viability in culture. While the SSV did not change the dermis thickness, the SF maintained the tissue proliferative potential, assessed by the nucleolar organizer region area, similar to noncryopreserved tissues. Moreover, both techniques reduced the number of fibroblasts and increased the percentage of collagen fibers. Nevertheless, only tissues cryopreserved with SF and noncryopreserved tissues were able to produce cells after in vitro culture. Although SF did not alter cell viability and proliferative activity, cells derived from cryopreserved tissues showed decreased metabolism, altered apoptosis, increased levels of reactive oxygen species, and mitochondrial membrane potential compared to cells from noncryopreserved tissues. In summary, we demonstrated for the first time that Antillean manatee somatic tissues can be cryopreserved by SF, and cells can be obtained after in vitro culture. Improvements in cryopreservation conditions, especially vitrification, of somatic samples are needed to increase the quality of somatic tissue banks in this species.
(© 2023 Wiley Periodicals LLC.)
Databáze: MEDLINE
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