Multi-omic approach to decipher the impact of skincare products with pre/postbiotics on skin microbiome and metabolome.

Autor:	Li M; Colgate-Palmolive Company, Piscataway, NJ, United States., Mao J; Colgate-Palmolive Company, Piscataway, NJ, United States., Diaz I; Colgate-Palmolive Company, Piscataway, NJ, United States., Kopylova E; Clarity Genomics Inc., San Diego, CA, United States.; Arome Science Inc., Farmington, CT, United States., Melnik AV; Clarity Genomics Inc., San Diego, CA, United States.; Arome Science Inc., Farmington, CT, United States., Aksenov AA; Clarity Genomics Inc., San Diego, CA, United States.; Arome Science Inc., Farmington, CT, United States., Tipton CD; RTL Genomics, MicroGenDX, Lubbock, TX, United States., Soliman N; Colgate-Palmolive Company, Piscataway, NJ, United States., Morgan AM; Colgate-Palmolive Company, Piscataway, NJ, United States., Boyd T; Colgate-Palmolive Company, Piscataway, NJ, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in medicine [Front Med (Lausanne)] 2023 Jul 18; Vol. 10, pp. 1165980. Date of Electronic Publication: 2023 Jul 18 (Print Publication: 2023).
DOI:	10.3389/fmed.2023.1165980
Abstrakt:	Introduction: Although pre/pro/postbiotics have become more prevalent in dermatologic and cosmetic fields, the mode of action when topically applied is largely unknown. A multi-omic approach was applied to decipher the impact of the skincare products with pre/postbiotics on skin microbiome and metabolome. Methods: Subjects with dry skin applied a body wash and body lotion with or without pre/postbiotics for 6 weeks. Skin hydration was measured at baseline, 3 and 6 weeks. Skin swabs were collected for 16S rRNA gene sequencing, metagenomics and metabolomics analysis. Results: Skin hydration significantly increased in both groups. The prebiotic group significantly reduced opportunistic pathogens, e.g., Pseudomonas stutzeri and Sphingomonas anadarae , and increased the commensals, e.g., Staphylococcus equorum , Streptococcus mitis, Halomonas desiderata . Bacterial sugar degradation pathways were enriched in the prebiotic group, while fatty acid biosynthesis pathways were reduced in control. The changes on skin metabolome profiles by the products were more prominent. The prebiotic group performed greater modulation on many clinically-relevant metabolites compared to control. Correlation analysis showed H. desiderata and S. mitis positively correlated with skin hydration, P. stutzeri and S. anadarae negatively correlated with the metabolites that are positively associated with skin hydration improvement. Conclusion: This holistic study supported a hypothesis that the pre/postbiotics increased skin hydration through the modulation of skin microbiome, metabolic pathways and metabolome. Competing Interests: This study received funding from Colgate-Palmolive. The funder had the following involvement with the study: clinical study design, multi-omics data analysis and interpretation, decision to publish and preparation of the manuscript. ML, ID, NS, AMM, TB, and JM are employees of Colgate-Palmolive. AA, AVM, and EK are consultants of Clarity Genomics, Inc., and Arome Science Inc., and were compensated by Colgate-Palmolive for their contributions to the mass spectrometry data acquisition and analysis. CT is the employee of RTL Genomics and was compensated by Colgate-Palmolive for the contribution to 16S sequencing analysis. (Copyright © 2023 Li, Mao, Diaz, Kopylova, Melnik, Aksenov, Tipton, Soliman, Morgan and Boyd.)
Databáze:	MEDLINE
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