LncRNA MALAT1 promotes osteogenic differentiation through the miR-93-5p/SMAD5 axis.

Autor: Gu Y; Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University, Beijing, China., Bai Y; Department of Orthodontics, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
Jazyk: angličtina
Zdroj: Oral diseases [Oral Dis] 2024 May; Vol. 30 (4), pp. 2398-2409. Date of Electronic Publication: 2023 Aug 03.
DOI: 10.1111/odi.14705
Abstrakt: Objectives: Promoting the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) is a way to regenerate periodontal bone. This study aimed to determine whether lncRNA MALAT1 promotes the osteogenic differentiation of human PDLSCs in vitro.
Materials and Methods: Human PDLSCs were extracted from the human periodontal ligament, and after osteogenic differentiation was induced using osteogenic medium, the human PDLSCs were transfected with siRNA-MALAT1, miR-93-5p mimics, and miR-93-5p inhibitors. The expression of osteogenesis-related genes was assessed by RT-qPCR and western blotting, alkaline phosphatase (ALP) activity was assessed by ALP activity assay, and the formation of mineralized nodules was assessed by alizarin red S (ARS) staining. RNA immunoprecipitation (RIP) and luciferase assays were performed to assess the binding of MALAT1, miR-93-5p, and SMAD5.
Results: The expression of lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) was upregulated, while that of miR-93-5p was downregulated after PDLSC osteogenic differentiation. Knockdown of MALAT1 inhibited the osteogenic differentiation of PDLSCs, and MALAT1 expression negatively correlated with miR-93-5p expression. miR-93-5p inhibited the osteogenic differentiation of human PDLSCs by specifically binding to SMAD5.
Conclusion: MALAT1 regulates human PDLSC differentiation by regulating the miR-93-5p/SMAD5 axis.
(© 2023 Wiley Periodicals LLC.)
Databáze: MEDLINE
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