Nanoscale details of mitochondrial constriction revealed by cryoelectron tomography.

Autor:	Mageswaran SK; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California; Department of Biophysics and Biochemistry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania; Institute of Structural Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania., Grotjahn DA; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California. Electronic address: grotjahn@scripps.edu., Zeng X; Computational Biology Department, School of Computer Science, Carnegie Mellon University, Pittsburgh, Pennsylvania., Barad BA; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California., Medina M; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California., Hoang MH; Department of Biophysics and Biochemistry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania., Dobro MJ; Hampshire College, Amherst, Massachusetts., Chang YW; Department of Biophysics and Biochemistry, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania; Institute of Structural Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania., Xu M; Computational Biology Department, School of Computer Science, Carnegie Mellon University, Pittsburgh, Pennsylvania., Yang WY; Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan., Jensen GJ; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California; Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah. Electronic address: grant_jensen@byu.edu.
Jazyk:	angličtina
Zdroj:	Biophysical journal [Biophys J] 2023 Sep 19; Vol. 122 (18), pp. 3768-3782. Date of Electronic Publication: 2023 Aug 01.
DOI:	10.1016/j.bpj.2023.07.030
Abstrakt:	Mitochondria adapt to changing cellular environments, stress stimuli, and metabolic demands through dramatic morphological remodeling of their shape, and thus function. Such mitochondrial dynamics is often dependent on cytoskeletal filament interactions. However, the precise organization of these filamentous assemblies remains speculative. Here, we apply cryogenic electron tomography to directly image the nanoscale architecture of the cytoskeletal-membrane interactions involved in mitochondrial dynamics in response to damage. We induced mitochondrial damage via membrane depolarization, a cellular stress associated with mitochondrial fragmentation and mitophagy. We find that, in response to acute membrane depolarization, mammalian mitochondria predominantly organize into tubular morphology that abundantly displays constrictions. We observe long bundles of both unbranched actin and septin filaments enriched at these constrictions. We also observed septin-microtubule interactions at these sites and elsewhere, suggesting that these two filaments guide each other in the cytosolic space. Together, our results provide empirical parameters for the architecture of mitochondrial constriction factors to validate/refine existing models and inform the development of new ones. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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