| Autor: |
Nguyen VHT; Traphaco Joint Stock Company.; Faculty of Medicine and Pharmacy, Dainam University., Do HT; National Institute of Medical Materials., Tran HT; Thai Binh University Medicine and Pharmacy., Vu HT; Traphaco Joint Stock Company., Nguyen TT; National Institute of Medical Materials., Vu DT; National Institute of Medical Materials., Nguyen HLT; National Institute of Medical Materials., Nguyen HV; Traphaco Joint Stock Company., Tran QL; Traphaco Joint Stock Company., Nguyen VAT; Traphaco Joint Stock Company. |
| Jazyk: |
angličtina |
| Zdroj: |
Biological & pharmaceutical bulletin [Biol Pharm Bull] 2023; Vol. 46 (8), pp. 1057-1064. |
| DOI: |
10.1248/bpb.b22-00892 |
| Abstrakt: |
Glinus oppositifolius is an endemic herbaceous plant found in tropical Asian countries and is native in Vietnam. It is used in traditional folk medicine because of its flavor and antiseptic and laxative effects. In the current research, the effects of Tox-off, Biovip, and the purified compounds isolated from G. oppositifolius in the previous study were evaluated on the activation of adenosine 5'-monophosphate-activated protein kinase (AMPK)-activated protein kinase (AMPK) and acetyl-coenzyme A carboxylase (ACC) in C2C12 myoblasts. In addition, the most potent active compounds, traphanoside-GO1 (TRA-GO1) and TRA-GO5 have validated the reduction of fatty acid synthase (FAS) and sterol regulatory element binding protein (SREBP)-1c in HepG2 cells. We found that Tox-off and Biovip significantly increased the phosphorylation of AMPK and ACC in C2C12 myoblasts. Furthermore, TRA-GO1 and TRA-GO5 significantly increased the AMPK activation and phosphorylation of its downstream substrate ACC in a concentration-dependent way compared to the dimethyl sulfoxide (DMSO) control. Besides, the protein level of FAS and SREBP-1c decreased by TRA-GO1 and TRA-GO5 in a concentration-dependent manner. Taken together, our results showed that the increased AMPK and ACC phosphorylation by active components of G. oppositifolius may activate the AMPK signaling pathways, which are useful for the anti-obesity and its related metabolic disorders. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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