Increasing the coverage of the N-terminome with LysN amino terminal enrichment (LATE).

Autor:	Hanna R; Faculty of Biology, Technion-Israel Institute of Technology, Technion City, Haifa, Israel., Rozenberg A; Faculty of Biology, Technion-Israel Institute of Technology, Technion City, Haifa, Israel., Lavy T; Faculty of Biology, Technion-Israel Institute of Technology, Technion City, Haifa, Israel., Kleifeld O; Faculty of Biology, Technion-Israel Institute of Technology, Technion City, Haifa, Israel. Electronic address: okleifeld@technion.ac.il.
Jazyk:	angličtina
Zdroj:	Methods in enzymology [Methods Enzymol] 2023; Vol. 686, pp. 1-28. Date of Electronic Publication: 2023 May 18.
DOI:	10.1016/bs.mie.2023.04.003
Abstrakt:	The field of N-terminomics has been advancing with the development of novel methods that provide a comprehensive and unbiased view of the N-terminome. Negative selection N-terminomics enables the identification of free and naturally modified protein N-termini. Here, we present a streamlined protocol that combines two negative selection N-terminomics methods, LATE and HYTANE, to increase N-terminome coverage by 1.5-fold compared to using a single methodology. Our protocol includes sample preparation and data analysis of both methods and can be applied to studying the N-terminome of diverse samples. The suggested approach enables researchers to achieve a more detailed and accurate understanding of the N-terminome. (Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu