Evaluation of motor and sensory neuron populations in a mouse median nerve injury model.

Autor: Topley M; Department of Surgery, Queen's University, Kingston, ON, Canada; Centre for Neuroscience Studies, Queen's University, Canada., Crotty AM; Department of Surgery, Queen's University, Kingston, ON, Canada; Centre for Neuroscience Studies, Queen's University, Canada., Boyle A; Centre for Neuroscience Studies, Queen's University, Canada., Peller J; Centre for Neuroscience Studies, Queen's University, Canada., Kawaja M; Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada; Centre for Neuroscience Studies, Queen's University, Canada., Hendry JM; Department of Surgery, Queen's University, Kingston, ON, Canada; Kingston Health Sciences Center, Kingston, ON, Canada; Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada; Centre for Neuroscience Studies, Queen's University, Canada. Electronic address: Michael.hendry@kingstonhsc.ca.
Jazyk: angličtina
Zdroj: Journal of neuroscience methods [J Neurosci Methods] 2023 Aug 01; Vol. 396, pp. 109937. Date of Electronic Publication: 2023 Jul 31.
DOI: 10.1016/j.jneumeth.2023.109937
Abstrakt: Background: Peripheral nerves can regenerate and restore function after injury but this process is hindered by many factors including chronic denervation, motor end-plate resorption and Schwann cell senescence. Forelimb injury models in rodents are becoming increasingly popular as they more accurately reflect the physiology and biomechanics of upper extremity nerve injuries. However several aspects of this surgical model remain poorly characterized.
New Method: C57Bl/6 mice underwent enumeration of median nerve motor and sensory neuron pools using retrograde labeling with or without nerve transection. Distal histomorphometry of uninjured mouse median nerves was also examined. Baseline reference values of volitional forelimb grip strength measurements were determined and the rate of neural elongation was also estimated.
Results: We identified 1363 ± 165 sensory and 216 ± 16 motor neurons within the uninjured dorsal root ganglia (DRG) and ventral spinal cord, respectively. Eight days following injury, approximately 34% of motoneurons had elongated a distance of 5 mm beyond the repair site 8 days following injury. Volitional grip strength decreased 50% with unilateral median nerve transection and was negligible with contralateral flexor tendon tenotomy.
Comparison With Existing Method: Our spinal cord and DRG harvesting technique presented here was technically straightforward and reliable. Estimates of motor and sensory neuron numbers for the mouse median nerve compared favourably with studies using intramuscular injection of retrograde neurotracer. Histomorphometry data was consistent with and reinforced reference values in the literature.
Conclusions: This study provides data that further develops an increasingly popular surgical model for studying peripheral nerve injury and repair.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: