IL-33 promotes sciatic nerve regeneration in mice by modulating macrophage polarization.

Autor: Wasman Smail S; Department of Medical Microbiology, College of Science, Cihan University-Erbil, Kurdistan Region, Iraq; Department of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq., Ziyad Abdulqadir S; Department of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq., Omar Khudhur Z; Department of Biology Education, Faculty of Education, Tishk International University - Erbil, Kurdistan Region, Iraq. Electronic address: zhikal.omer@tiu.edu.iq., Elia Ishaq S; Department of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq., Faqiyazdin Ahmed A; KBMS, College of Medicine, Hawler Medical University, Erbil, Kurdistan Region, Iraq., Ghayour MB; Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran., Abdolmaleki A; Department of Biophysics, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran. Electronic address: Abdolmalekiarash1364@uma.ac.ir.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2023 Oct; Vol. 123, pp. 110711. Date of Electronic Publication: 2023 Jul 31.
DOI: 10.1016/j.intimp.2023.110711
Abstrakt: Despite the innate regenerative capacity of peripheral nerves, regeneration after a severe injury is insufficient, and sensorimotor recovery is incomplete. As a result, finding alternative methods for improving regeneration and sensorimotor recovery is essential. In this regard, we investigated the effect of IL-33 treatment as a chemokine with neuroprotective properties. IL-33 can facilitate tissue healing by potentiating the type 2 immune response and polarizing macrophages toward the pro-healing M2 phenotype. However, its effects on nerve regeneration remain unclear. Therefore, this research aimed to evaluate the neuroprotective effects of IL-33 on sciatic nerve injury in male C57BL/6 mice. After crushing the left sciatic nerve, the animals were given 10, 25, or 50 µg/kg IL-33 intraperitoneally for seven days. The sensorimotor recovery was then assessed eight weeks after surgery. In addition, immunohistochemistry, ELISA, and real-time PCR were used to assess macrophage polarization, cytokine secretion, and neurotrophic factor expression in the injured nerves. IL-33 at 50 and 25 µg/kg doses could significantly accelerate nerve regeneration and improve sensorimotor recovery when compared to 10 µg/kg IL-33 and control groups. Furthermore, at 50 and 25 µg/kg doses, IL-33 polarized macrophages toward an M2 phenotype and reduced proinflammatory cytokines at the injury site. It also increased the mRNA expression of NGF, VEGF, and BDNF. These findings suggest that a seven-day IL-33 treatment had neuroprotective effects in a mouse sciatic nerve crush model, most likely by inducing macrophage polarization toward M2 and regulating inflammatory microenvironments.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: