| Autor: |
Gonzalez Lopez M; Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Huteckova B; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Brno, Czech Republic., Lavicky J; Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Zezula N; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic., Rakultsev V; Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Fridrichova V; Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Tuaima H; Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic., Nottmeier C; Department of Orthodontics, University of Leipzig Medical Center, Leipzig, Germany., Petersen J; Department of Orthodontics, University of Leipzig Medical Center, Leipzig, Germany., Kavkova M; Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic., Zikmund T; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic., Kaiser J; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic., Lav R; Centre for Craniofacial and Regenerative Biology, King's College London, London, UK., Star H; Centre for Craniofacial and Regenerative Biology, King's College London, London, UK., Bryja V; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic., Henyš P; Institute of New Technologies and Applied Informatics, Faculty of Mechatronics, Informatics and Interdisciplinary Studies, Technical University of Liberec, Liberec, Czech Republic., Vořechovský M; Institute of Structural Mechanics, Faculty of Civil Engineering, Brno University of Technology, Czech Republic., Tucker AS; Centre for Craniofacial and Regenerative Biology, King's College London, London, UK.; Institute of Histology and Embryology, First Faculty of Medicine, Charles University, Prague, Czech Republic., Harnos J; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic., Buchtova M; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.; Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Brno, Czech Republic., Krivanek J; Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. |
| Abstrakt: |
Mineralized tissues, such as bones or teeth, are essential structures of all vertebrates. They enable rapid movement, protection, and food processing, in addition to providing physiological functions. Although the development, regeneration, and pathogenesis of teeth and bones have been intensely studied, there is currently no tool to accurately follow the dynamics of growth and healing of these vital tissues in space and time. Here, we present the BEE-ST (Bones and tEEth Spatio-Temporal growth monitoring) approach, which allows precise quantification of development, regeneration, remodeling, and healing in any type of calcified tissue across different species. Using mouse teeth as model the turnover rate of continuously growing incisors was quantified, and role of hard/soft diet on molar root growth was shown. Furthermore, the dynamics of bones and teeth growth in lizards, frogs, birds, and zebrafish was uncovered. This approach represents an effective, highly reproducible, and versatile tool that opens up diverse possibilities in developmental biology, bone and tooth healing, tissue engineering, and disease modeling. |
