| Autor: |
Kohn EM; Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States., Konovalov K; Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.; Theoretical Chemistry Institute, Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States., Gomez CA; Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States., Hoover GN; Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States., Yik AK; Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.; Theoretical Chemistry Institute, Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States., Huang X; Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.; Theoretical Chemistry Institute, Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States., Martell JD; Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.; Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53705, United States. |
| Abstrakt: |
Nucleic acid-based receptors, known as aptamers, are relatively fast to discover and manufacture but lack the diverse functional groups of protein receptors (e.g., antibodies). The binding properties of DNA aptamers can be enhanced by attaching abiotic functional groups; for example, aromatic groups such as naphthalene slow dissociation from proteins. Although the terminal alkyne is a π-electron-rich functional group that has been used in small molecule drugs to enhance binding to proteins through noncovalent interactions, it remains unexplored for enhancing DNA aptamer binding affinity. Here, we demonstrate the utility of the terminal alkyne for improving the binding of DNA to proteins. We prepared a library of 256 terminal-alkyne-bearing variants of HD22, a DNA aptamer that binds the protein thrombin with nanomolar affinity. After a one-step thrombin-binding selection, a high-affinity aptamer containing two alkynes was discovered, exhibiting 3.2-fold tighter thrombin binding than the corresponding unmodified sequence. The tighter binding was attributable to a slower rate of dissociation from thrombin (5.2-fold slower than HD22). Molecular dynamics simulations with enhanced sampling by Replica Exchange with Solute Tempering (REST2) suggest that the π-electron-rich alkyne interacts with an asparagine side chain N-H group on thrombin, forming a noncovalent interaction that stabilizes the aptamer-protein interface. Overall, this work represents the first case of terminal alkynes enhancing the binding properties of an aptamer and underscores the utility of the terminal alkyne as an atom economical π-electron-rich functional group to enhance binding affinity with minimal steric perturbation. |
