Immunomodulatory effects of canine mesenchymal stem cells in an experimental atopic dermatitis model.

Autor: Kang SJ; Viral Diseases Research Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea., Gu NY; Viral Diseases Research Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea., Byeon JS; Viral Diseases Research Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea., Hyun BH; Viral Diseases Research Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea., Lee J; Division of Regenerative Medicine Safety Management, Department of Chronic Disease Convergence Research, Korea National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju, Republic of Korea., Yang DK; Viral Diseases Research Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea.
Jazyk: angličtina
Zdroj: Frontiers in veterinary science [Front Vet Sci] 2023 Jul 13; Vol. 10, pp. 1201382. Date of Electronic Publication: 2023 Jul 13 (Print Publication: 2023).
DOI: 10.3389/fvets.2023.1201382
Abstrakt: Mesenchymal stem cells (MSCs) have the potential to differentiate into multi-lineage cells, suggesting their future applicability in regenerative medicine and biotechnology. The immunomodulatory properties of MSCs make them a promising replacement therapy in various fields of animal research including in canine atopic dermatitis (AD), a skin disease with 10-15% prevalence. We investigated the immunomodulatory effects of MSCs in an experimental canine AD model induced by Dermatophagoides farinae extract ointment. Canine adipose tissue-derived MSCs (cAT-MSCs) were differentiated into mesodermal cell lineages at the third passage. Alterations in immunomodulatory factors in control, AD, and MSC-treated AD groups were evaluated using flow cytometric analysis, enzyme-linked immunosorbent assay, and quantitative reverse transcription PCR. In the MSC-treated AD group, the number of eosinophils decreased, and the number of regulatory T cells (Tregs) increased compared to those in the AD group. In addition, the immunoglobulin E (IgE) and prostaglandin E 2 levels were reduced in the MSC-treated AD group compared to those in the AD group. Furthermore, the filaggrin, vascular endothelial growth factor, and interleukin-5 gene expression levels were relatively higher in the MSC-treated AD group than in the AD group, however, not significantly. cAT-MSCs exerted immunomodulatory effects in an AD canine model via a rebalancing of type-1 and -2 T helper cells that correlated with increased levels of Tregs, IgE, and various cytokines.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Kang, Gu, Byeon, Hyun, Lee and Yang.)
Databáze: MEDLINE
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