Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice.

Autor:	Immormino RM; Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United States., Smeekens JM; Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United States.; UNC Food Allergy Initiative, Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United States., Mathai PI; Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United States., Kesselring JR; Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United States.; UNC Food Allergy Initiative, Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United States., Turner AV; Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United States.; UNC Food Allergy Initiative, Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United States., Kulis MD; Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United States.; UNC Food Allergy Initiative, Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United States., Moran TP; Department of Pediatrics, UNC School of Medicine, Chapel Hill, NC, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in allergy [Front Allergy] 2023 Jul 17; Vol. 4, pp. 1219268. Date of Electronic Publication: 2023 Jul 17 (Print Publication: 2023).
DOI:	10.3389/falgy.2023.1219268
Abstrakt:	Background: Early dietary introduction of peanut has shown efficacy in clinical trials and driven pediatric recommendations for early introduction of peanut to children with heightened allergy risk worldwide. Unfortunately, tolerance is not induced in every case, and a subset of patients are allergic prior to introduction. Here we assess peanut allergic sensitization and oral tolerance in genetically diverse mouse strains. Objective: We aimed to determine whether environmental adjuvant-driven airway sensitization and oral tolerance to peanut could be induced in various genetically diverse mouse strains. Methods: C57BL/6J and 12 Collaborative Cross (CC) mouse strains were fed regular chow or ad libitum peanut butter to induce tolerance. Tolerance was tested by attempting to sensitize mice via intratracheal exposure to peanut and lipopolysaccharide (LPS), followed by intraperitoneal peanut challenge. Peanut-specific immunoglobulins and peanut-induced anaphylaxis were assessed. Results: Without oral peanut feeding, most CC strains (11/12) and C57BL/6J induced peanut-specific IgE and IgG1 following airway exposure to peanut and LPS. With oral peanut feeding none of the CC strains nor C57BL/6J mice became sensitized to peanut or experienced anaphylaxis following peanut challenge. Conclusion: Allergic sensitization and oral tolerance to peanut can be achieved across a range of genetically diverse mice. Notably, the same strains that became allergic via airway sensitization were tolerized by feeding high doses of peanut butter before sensitization, suggesting that the order and route of peanut exposure are critical for determining the allergic fate. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (© 2023 Immormino, Smeekens, Mathai, Kesselring, Turner, Kulis and Moran.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu