Divergence of trafficking and polarization mechanisms for PIN auxin transporters during land plant evolution.

Autor:	Tang H; Institute of Science and Technology Austria (ISTA), Am Campus 1, 3400 Klosterneuburg, Austria., Lu KJ; Graduate Institute of Biotechnology, National Chung Hsing University, No. 145, Xingda Rd., South Dist., Taichung 40227, Taiwan, R.O.C., Zhang Y; College of Life Sciences, Northwest A&F University, Shaanxi, Yangling, China., Cheng YL; Institute of Plant and Microbial Biology, Academia Sinica, 128 Sec. 2, Academia Rd, Nankang, Taipei 11529, Taiwan, R.O.C., Tu SL; Institute of Plant and Microbial Biology, Academia Sinica, 128 Sec. 2, Academia Rd, Nankang, Taipei 11529, Taiwan, R.O.C., Friml J; Institute of Science and Technology Austria (ISTA), Am Campus 1, 3400 Klosterneuburg, Austria. Electronic address: jiri.friml@ist.ac.at.
Jazyk:	angličtina
Zdroj:	Plant communications [Plant Commun] 2024 Jan 08; Vol. 5 (1), pp. 100669. Date of Electronic Publication: 2023 Jul 31.
DOI:	10.1016/j.xplc.2023.100669
Abstrakt:	The phytohormone auxin, and its directional transport through tissues, plays a fundamental role in the development of higher plants. This polar auxin transport predominantly relies on PIN-FORMED (PIN) auxin exporters. Hence, PIN polarization is crucial for development, but its evolution during the rise of morphological complexity in land plants remains unclear. Here, we performed a cross-species investigation by observing the trafficking and localization of endogenous and exogenous PINs in two bryophytes, Physcomitrium patens and Marchantia polymorpha, and in the flowering plant Arabidopsis thaliana. We confirmed that the GFP fusion did not compromise the auxin export function of all examined PINs by using a radioactive auxin export assay and by observing the phenotypic changes in transgenic bryophytes. Endogenous PINs polarize to filamentous apices, while exogenous Arabidopsis PINs distribute symmetrically on the membrane in both bryophytes. In the Arabidopsis root epidermis, bryophytic PINs have no defined polarity. Pharmacological interference revealed a strong cytoskeletal dependence of bryophytic but not Arabidopsis PIN polarization. The divergence of PIN polarization and trafficking is also observed within the bryophyte clade and between tissues of individual species. These results collectively reveal the divergence of PIN trafficking and polarity mechanisms throughout land plant evolution and the co-evolution of PIN sequence-based and cell-based polarity mechanisms. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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